实验性视网膜脱离及复位后视网膜色素上皮细胞抗增殖性细胞核抗原的表达  

Investigation of the expression of proliferating cell nuclear antigen of retinal pigment epithelial cells after experimental retinal detachment and reattachment in cats

作　　者：刘铁城[1] 马志中[1] 张卯年[1] 刘百臣[1] 师锁柱[2] 王常观[1] 张高魁[3] 

机构地区：[1]北京解放军总医院眼科,100853 [2]北京解放军总医院肾科实验室,100853 [3]北京解放军总医院统计教研室,100853

出　　处：《中华眼底病杂志》2003年第1期20-23,共4页Chinese Journal of Ocular Fundus Diseases

摘　　要：目的　观察视网膜脱离和复位过程中抗增殖性细胞核抗原 (proiferating cell nuclear anti-gen,PCNA)表达的状况 ,以评价其在接触抑制机制中的意义。　方法　对 72只猫眼行晶状体囊外摘除和玻璃体切除手术。3周后 ,利用微穿刺技术制作视网膜脱离和复位动物模型 ,在不同时间取眼球并制成组织切片。采用免疫组织化学方法 ,观察视网膜脱离后视网膜色素上皮 (retinal pigment epithelium ,RPE)细胞PCNA的表达。光镜下确定 PCNA阳性的 RPE细胞并量化增殖反应。采用方差分析 (ANOVA)方法 ,对脱离组的脱离区和非脱离区及复位组复位区 RPE细胞层 PCNA的表达水平进行比较。　结果　在视网膜脱离后 2 4 h,脱离组脱离区视网膜 RPE细胞层出现 PCNA阳性细胞 ,5～ 6 d达到高峰 ,2 0 d后逐渐回落。在复位组复位区 ,PCNA标记的 RPE细胞明显少于脱离组脱离区。脱离组非脱离区视网膜很少有 PCNA标记的 RPE细胞。 3组 PCNA标记的 RPE细胞数比较 ,差异有显著性的意义。　结论　视网膜脱离诱导RPE细胞增殖 ,而视网膜复位后这种增殖受到抑制。表明视网膜复位过程中 ,接触抑制的机制在发挥作用。Objective To examine the expression of proliferating cell nuclear antigen (PCNA) of retinal pigment epithelial (RPE) cells, thus assessing the role of mechanism of contact inhibition playing in the process of experimental retinal detachment and reattachemnt. Methods Retinal detachment was produced in 72 cats by subretinal injection of 0.25% solution of healon through a micropipette three weeks after extracapsular lens extraction and vitrectomy. Some of the detached retinae were reattached 24 hours later. At different time, the cats were killed and eye globes were fixed and embeded in paraffin. Histologic sections were processed for immunohistochemistry examination using an antibody to detect PCNA protein. Labeled RPE cells were identified, and the proliferation was quantified in detached and un detached retinae of detachment group, and also in reattached retinae of reattachment group. The comparsion of PCNA labeled RPE cells in different groups were analyzed by ANOVA. Results In detached regions of detachment group, PCNA expression of RPE cells occured within 24 hours, and reached a maximum after 5 6 days, then gradually declined to barely detectable levels after 20 days. Similar tendency was found in reattached retinae, but the number of PCNA labeled RPE cells was obviously small. Fewer PCNA labeled RPE cells were found in regions of un detached retinae in detachment group. The difference of these three groups was significant. Conclusion Proliferation of RPE cells is induced when they lose contact with neural retina, but inhibited after neural retina reattached to RPE cells. It suggests that the mechanism of contact inhibition plays a role in the proliferative process after retinal detachment and reattachment.

关 键 词：视网膜 复位 增殖细胞核抗原 色素上皮 接触抑制 视网膜脱离 

分 类 号：R774.1[医药卫生—眼科]