热休克蛋白70、90在子宫内膜癌中的表达  

Expression and significance of heat shock protein 70, 90 in endometrial carcinomas

作　　者：白晓霞[1] 陈亚琼[1] 辛晓燕[1] 王文栋[2] 

机构地区：[1]第四军医大学西京医院妇产科,陕西西安710032 [2]解放军第106医院妇产科,山东济南250022

出　　处：《细胞与分子免疫学杂志》2003年第1期38-40,共3页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的:探讨子宫内膜癌组织中热休克蛋白(HSP)70、90的表达及意义。方法:用免疫组化Envision法及图象分析仪,检测 30例正常子宫内膜、30例增生过长子宫内膜和53例子宫内膜癌中HSP70、HSP90的表达。结果:子宫内膜癌中HSP70表达的灰度值为(209.06±5.36),明显高于正常内膜[(145.21±4.09),P<0.01]和增生过长内膜[(148.59±4.23),P<0.01];子宫内膜癌中HSP90表达的灰度值为(166.98±5.71),明显低于正常子宫内膜[(208.57±31.14),P<0.05]和增生过长子宫内膜[(249.73±4.94),P<0.01]。子宫内膜癌中HSP70的表达随肿瘤病理分级的增加而增强(P<0.01),非内膜样癌(229.90±3.77)较内膜样癌表达强[(198.37±3.15),P<0.01];子宫内膜癌中HSP90表达随肿瘤病理分级的升高而表达减弱,非内膜样癌(140.21±3.22)较内膜样癌表达减弱[(176.59±2.79),P<0.01]。子宫内膜癌中HSP70、90的表达与肌层浸润深度、临床分期及淋巴结转移未见显著相关性(P>0.05)。结论:HSP70、90可能与子宫内膜癌的发生及预后有关。AIM: To explore the expression and significance of heat shock protein ( HSP) 70 ,90 in endometrial carcinomas. METHODS; Expresssions of HSP70 and HSP90 in 30 normal endometrium, 30 endometrial hyperplasia and 53 endometrial carcinomas were detected by Envision immunohisto-chemical staining and imaging pattern analyser. RESULTS : Gray Scale value (GS) of HSP70 in endometrial carcinoma being (209.06±5.36) GS was obviously higher than that in normal endometrium (145.21 ±4.09)GS, P<0.01 and endometrial hyperplasia (148. 59 ± 4. 23) GS, P<0.01. GS of HSP90 in endometrial carcinoma being (166. 98 ± 5. 71) GS was markedly lower than that in normal endometrium (208.57 ±3.14)GS, P<0.05 and endometrial hyperplasia (249.73 ±4.94)GS, P<0.01. Expression of HSP70 increased with increased pathological grading of tumor P<0.01, whereas HSP90 expression decreased with increased pathological grading P<0.01. Expression of HSP70 in nonendometrioid carcinoma (229.90 ± 3. 77) GS was higher than in endom-etrioid carcinoma (198.37±3.15)GS, P<0.01 and the expression pattern of HSP90( 140. 21±3. 22) was contrary to that of HSP70 (176.59±2.79)GS, P<0.01. There was no correlations among expression of HSP70, 90 in endometrial carcinoma and depth invaded into muscular layer, clinical stage and lymph node metastasis. CONCLUSION: Both HSP70 and HSP90 may have relation to the genesis and prognosis of endometrial carcinoma.

关 键 词：热休克蛋白70 子宫内膜癌 免疫组织化学 

分 类 号：R737.33[医药卫生—肿瘤]