LDL-ACM复合物对裸鼠皮下移植瘤靶向抑制作用的初步研究  

Inhibitory effects of LDL-ACM complex on subcutaneous implanted tumors in nude mice

作　　者：姜安丽[1] 康鲁东[1] 赵春华[1] 毕文祥[1] 胡晓燕[1] 孔峰[1] 徐松德[1] 

机构地区：[1]山东大学医学院生物化学教研室,山东济南250012

出　　处：《中国病理生理杂志》2003年第1期55-58,共4页Chinese Journal of Pathophysiology

基　　金：山东省科委基金资助项目 (No .971 1 6460 7)

摘　　要：目的 :通过观察比较LDL -ACM复合物和游离ACM对胃癌SGC - 790 1 ,NKM - 4 5细胞株裸鼠皮下移植的抑制效应 ,从而了解LDL作为抗癌药物靶向载体的应用价值。方法 :首先采用温育交换法制备LDL -ACM复合物 ,然后建立人胃癌细胞株裸鼠皮下移植瘤模型 ,以瘤重、肿瘤体积、白细胞计数、抑瘤率、生命延长率等指标观察LDL -ACM对移植瘤的抑制作用。结果 :LDL -ACM组的移植瘤生长速度明显慢于生理盐水组及游离ACM组 ,抑瘤率和生命延长率明显高于生理盐水组及游离ACM组 ,外周白细胞计数无明显变化 ,尤其对SGC - 790 1瘤更为明显。结论 :LDL -ACM复合物与游离ACM相比有更强的抑癌效应。LDLAIM: In order to evaluate the applicable value of LDL as a targeted vehicle for chemotherapeutic agents, we investigated and compared the inhibitory effects of LDL-ACM complex and free ACM on nude mice's subcutaneous implanted tumors derived from gastric cancer cell lines, SGC-7901 and NKM-45. METHODS: LDL-ACM complex was prepared and the tumor model of nude mice was established by subcutaneous implantation of SGC-7901 and NKM-45. Then, the groups of nude mice developed subcutaneous implanted tumors were received either LDL-ACM complex or free ACM. Subsequently, the tumor size, weight and leukemia cell counts were measured and the rates of tumor-inhibition and the survival were compared among the groups. RESULTS: The inhibitory effects of LDL-ACM complex on the tumors, especially on SGC-7901 implanted tumors were much more obvious than that of free ACM. It was also indicated that the action of LDL-ACM complex was mediated by LDL receptor. CONCLUSION: These results showed that LDL-ACM complex had significant inhibitory effects on the implanted tumors and the effect might be mediated by LDL receptor.

关 键 词：LDL-ACM复合物 皮下移植瘤 脂蛋白类 阿柔比星 胃肿瘤 细胞株 

分 类 号：R735.2[医药卫生—肿瘤]