大豆KUNITZ型胰蛋白酶抑制剂的稳定性及抗虫性研究  

A Study of the Stability and Insect Resistance of Soybean Kuntiz-type Trypsin Inhibitor

作　　者：谢可方[1] 董爱武[1] 忻骅[1] 詹树萱[1] 丁波[1] 顾其敏[1] 曹凯鸣[1] 

机构地区：[1]复旦大学生命科学学院生物化学系,上海200433

出　　处：《复旦学报（自然科学版）》2002年第6期631-634,共4页Journal of Fudan University：Natural Science

基　　金：国家自然科学基金资助项目(39770083);国家自然科学基金重点资助项目(39730330)

摘　　要：大豆kunitz型胰蛋白酶抑制剂(SBTi A2)存在于种子中,2种kunitz型胰蛋白酶抑制剂Tia和Tid得到纯化,并对它们的pH稳定性和温度稳定性进行了比较研究,而且通过人工饲料喂养鳞翅目昆虫棉铃虫来研究SBTi A2的抗虫性.结果显示,棉铃虫的幼虫喂食含有Tia的人工饲料后,表现出生长延缓、体重减轻、羽化率降低、死亡率增加.由此显示了SBTi A2作为生物农药及利用转基因方法使作物提高抗虫性的应用前景.Soybean kuntiztype trypsin inhibitors (SBTi) are ubiquitous in soybean seeds and two kinds of SBTi- Tia and Tid were purified from different varieties of soybean seeds. Compare the pH stability and thermal stability of Tia and Tid then study the insectresistance of Tia by feeding Heliothis armgera Hubner, a kind of lepidopteran pest harmful to cotton. Heliothis armgera larvae which grow on artificial feed of Tia showed slow growth, light weight, low maturation, and high death rate, which indicates the further prospect of using SBTi as a biological chemical or as an insectresistant gene to be transferred into plants.

关 键 词：大豆胰蛋白酶抑制剂 稳定性 抗虫性 生物农药 

分 类 号：S482.39[农业科学—农药学]