甲基绿-派洛宁-马休黄组合染色法检测神经元凋亡  

The Method of MG-P-MY Staining in Detecting the Neuronal Apoptosis

作　　者：周建光[1] 刘长云[1] 黄亚莉[1] 刘景昌[2] 龚志锦[3] 詹洲 

机构地区：[1]解放军第四一一医院,上海200081 [2]海军医学研究所,上海200433 [3]第二军医大学,上海200433

出　　处：《海军医学杂志》2002年第4期289-290,共2页Journal of Navy Medicine

摘　　要：目的 :提供一种研究神经元凋亡的形态学方法。方法 :应用甲基绿 -派洛宁 -马休黄组合染色法 ,检测沙土鼠脑缺血后海马神经元的凋亡。结果及结论 :甲基绿 -派洛宁 -马休黄组合染色法能显示细胞质mRNA明显增高时期的凋亡神经元。Objective: To offer a new method in detecting neuronal apoptosis. Methods: The neuronal apoptosis in CA 1 region of hippocampus following ischemia reperfusion was detected using the MG P MY staining method which combined methyl green, pyronine and martius yellow. Results and Conclusion: The method of MG P MY staining can identify neuronal apoptosis only when mRNA is significantly increased in neuronal cytoplasm.

关 键 词：甲基绿-派洛宁-马休黄染色 神经元凋亡 形态学 沙土鼠 脑缺血 

分 类 号：R743.31[医药卫生—神经病学与精神病学] R446.1[医药卫生—临床医学]