豚鼠耳蜗单离Hensen细胞的分离技术及其钾通道  

Isolation of Hensen Cells From the Guinea-Pig Cochlea and the Properties of Potassium Currents in Isolating Hensen Cells

作　　者：李建雄[1] 郑建全[2] 程晓华[1] 王永安[2] 李兴启[1] 

机构地区：[1]解放军总医院耳鼻咽喉科研究所,北京100853 [2]解放军军事医学科学院

出　　处：《听力学及言语疾病杂志》2003年第1期32-34,I002,共4页Journal of Audiology and Speech Pathology

基　　金：国家自然科学基金资助项目 (编号 30 0 70 812 )

摘　　要：目的　探讨适合膜片钳技术的单离Hensen细胞的分离及对其钾通道的研究。方法　取豚鼠耳蜗 ,获得Corti器 ,采用酶消化和机械分离方法对Hensen细胞进行分离 ,并采用传统全细胞膜片钳技术 ,记录单离Hensen细胞的钾电流。结果　每只耳蜗可以获得活性良好的单离Hensen细胞 12± 3个。Hensen细胞的钾电流呈明显的外向整流性 ,只有迟电流 (IK) ,没有峰电流 (IA)。结论　酶消化结合机械分离的方法可获得适用于膜片钳技术研究的单离Hensen细胞 。Objective To probe the method of isolating Hensen cells for patch-clamp technique and study potassium currents in isolated Hensen cells. Methods The organs of Corti of the guinea-pig cochlea were treated using enzyme, and then dissected. The whole cell patch-clamp technique was used to record potassium currents in Hensen cells. Results The number of living Hensen cells of one cochlea that was obtained was 12±3. The outward K+ currents was delayed rectified, TEA-sensitive potassium currents (I K), not rapidly inactive, 4-AP-sensitive potassium currents (IA).Conclusion Isolated Hensen cells for patch clamp are gained by enzyme digesting and mechanical dispersing. The properties of potassium currents in isolated Hensen cells are discussed.

关 键 词：Hensen细胞 细胞分离 钾 膜片钳 

分 类 号：R764.3[医药卫生—耳鼻咽喉科]