高效液相色谱法测定牛奶中氯霉素残留量的研究  

Study on the rapid determination of chloramphenicol residues in milk by HPLC

作　　者：蒋定国 杨大进 方从容 文玉雪[2] 王玉莲[3] 王竹天 

机构地区：[1]中国疾控中心营养与食品安全所,北京100021 [2]珲春市卫生防疫站,吉林珲春133300 [3]泰安市卫生防疫站,山东泰安271000

出　　处：《中国食品卫生杂志》2003年第1期36-38,共3页Chinese Journal of Food Hygiene

基　　金：科技部社会公益研究专项资金项目~~

摘　　要：为了快速、简便、灵敏地测定牛奶中的氯霉素残留 ,建立了高效液相色谱分析法。牛奶样品先用含有 1%高氯酸的乙酸乙酯沉淀蛋白质和提取氯霉素 ,提取液浓缩干后用 0 5mol L高氯酸溶液溶解 ,以正己烷去除脂溶性杂质 ,在波长 2 78nm处用高效液相色谱仪测定氯霉素。本方法最低检出浓度为 1 1μg kg ,氯霉素浓度在 2 0 μg kg～ 10 0 μg kg范围内 ,方法平均回收率为 88 0 %～ 97 7% ,RSD为 5 3%～ 6 4 %。本方法适于牛奶中氯霉素残留的监测。A rapid,simple and sensitive high performance liquid chromatographic method for the determination of chloramphenicol residues in milk has been established.The protein in milk was first precipitated by 1% HClO\-4 in ethyl acetate,then the chloramphenicol was extracted by ethyl acetate.The residues was cleaned up by 0.5 mol/L HClO\-4 and n-hexane.The purified solution was examined at UV 278 nm by HPLC.The minimum detectable limit of this method was 1.1 μg/kg,the recovery rate was 88.0%～97.7%,and the RSD was 5.3%～6.4%.

关 键 词：高效液相色谱法 测定 牛奶 氯霉素残留量 兽医药 

分 类 号：R155.5[医药卫生—营养与食品卫生学]