肝癌细胞凋亡和增殖与CT表现的相关研究  

Correlation Studies Between the Apoptosis, the Proliferation and CT Images in Hepatocellular Carcinoma

作　　者：于继徐[1] 王滨[1] 高志芹[2] 史英红[1] 孙业全[1] 曹贵文[1] 

机构地区：[1]潍坊医学院附属医院影像中心,山东潍坊261031 [2]潍坊医学院细胞生物学教研室

出　　处：《实用放射学杂志》2003年第1期1-4,共4页Journal of Practical Radiology

基　　金：山东省教育厅资助课题 (编号J99K58);山东省科学技术委员会攻关项目资助课题 (项目编号 1 998BB1CCA1 )部分内容

摘　　要：目的　探讨肝细胞癌 (hepatocellularcarcinoma ,HCC)细胞凋亡 (apoptosis)和增殖与CT表现的关系。方法　手术切除并经病理证实的肝细胞癌 42例 ,所有病例均行螺旋CT平扫和动、静脉双期增强扫描。采用TdT缺口末端标记法 (TUNEL)和免疫组织化学SP法分别检测各标本的AI和PCNA -LI。结果　随着肿瘤的增大 ,凋亡指数 (Apoptosisindex ,AI)降低 ,PCNA -LI增高 (Ρ <0 .0 5 )。肿瘤包膜完整组AI高于无包膜或包膜欠完整组 (Ρ <0 .0 5 ) ,而增殖指数 (PCNA -Labelingindex ,PCNA -LI)在肿瘤包膜完整组低于无包膜或包膜欠完整组 (Ρ <0 .0 5 )。AI在门静脉无癌栓组高于门静脉有癌栓组 (Ρ <0 .0 1) ,PCNA -LI在门静脉无癌栓组低于门静脉有癌栓组 (Ρ <0 .0 1)。无淋巴结转移组中 ,AI较淋巴结转移组高 ,而PCNA -LI低于淋巴结转移组 (Ρ <0 .0 1)。AI和PCNA -LI呈负相关关系 (Ρ <0 .0 1)。结论　HCC的SCT表现与细胞凋亡和PCNA存在较为密切的相关关系 ,根据肝细胞癌的CT表现 。Objective To investigate the correlation between apoptosis, proliferation and spiral CT (SCT) images in hepatocellular carcinoma (HCC). Methods Forty-two HCC cases treated with surgical resection alone were studied. Plain and dual-phase enhanced SCT scanning was performed in all patients. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) and immunohistochemistry method were used to observe the apoptosis and PCNA. Results With HCC enlarging, apoptosis index (AI) decreased, and proliferating cell nuclear antigen-Labeling index (PCNA-LI) increased (Ρ<0.05).HCC with integral envelope, no portal vein infiltration and no lymph node metastasis had a significantly higher AI than those with portal vein infiltration, lymph node metastasis and without integral envelope (Ρ<0.05), but PCNA-LI was found in negative relation to AI on the above state (Ρ<0.05). AI was highly inversely related to PCNA-LI (Ρ<0.01). Conclusion The SCT images of HCC were closely associated with the AI, PCNA-LI. According to the SCT images of HCC, we could evaluate its AI and PCNA-LI.

关 键 词：肝癌 细胞凋亡 CT表现 细胞增殖 增殖细胞核抗原 

分 类 号：R735.7[医药卫生—肿瘤] R730.44[医药卫生—临床医学]