L-精氨酸防治大鼠高肺血流量所致肺动脉高压的研究  

Preventive and Therapeutic Effects of L-Arginine on Pulmonary Hypertension Induced by High Pulmonary Blood Flow Volume in Rats

作　　者：杜军保[1] 齐建光[1] 魏冰[1] 李简[1] 

机构地区：[1]北京大学第一医院,北京市100034

出　　处：《中国药房》2003年第1期13-14,共2页China Pharmacy

基　　金：国家自然科学基金资助项目 (30070796) ;卫生部科学研究基金资助项目 (98-1-261) ;北京市自然科学基金资助项目 (7012021)

摘　　要：目的 :研究L -精氨酸对高肺血流量所致肺动脉高压的防治作用。方法 :将雄性SD大鼠随机分为对照组 (n=6)、分流组(n=7)和分流 +L -精氨酸组 (n=8)。以腹主动脉 -下腔静脉分流术造成大鼠左向右分流。11周后 ,以右心导管法测定肺动脉平均压 (mPAP) ,并观测肺血管结构及平滑肌细胞PCNA的表达。结果 :分流组大鼠mPAP明显高于对照组 (P均<0 01) ,并且分流组大鼠肺小血管肌化程度明显增强 ,肺中、小肌型动脉相对中膜面积明显增加。11周分流组大鼠肺中、小型动脉平滑肌细胞PCNA的表达明显高于11周对照组大鼠 (P<0 05)。L -精氨酸缓解了肺动脉高压及肺血管结构重建的形成。结论 :L -精氨酸对高肺血流量所致肺血管结构重建及肺动脉高压有重要的防治作用。OBJECTIVE:To study the preventive and therapeutic actions of L-arginine on pulmonary hypertension induced by high pulmonary blood flow volume.METHODS:Male SD rats were randomly divided into control group(n=6),shunt group (n=7) and shunt+L-arginine group(n=8).Abdominal aorta-inferior vena cava shunt was performed to make the blood flow from left to right,11 weeks later,mPAP was detected with right cardiac catheter;the structure of pulmonary vessels and expression of PCNA of smooth muscle cells were observed.RESULTS:The mPAP of shunt group was obviously higher than that of control(P<0.01);the organization of pulmonary small vessels was obvious and relative area of tunica media of pulmonary middle and small arteries markedly increased;expression of PCNA of smooth muscle cell of pulmonary middle and small arteries was obviously higher than that of control (P<0.05).L-arginine slowed down pulmonary hypertension and reconstruction of pulmonary vessels.CONCLUSION:L-arginine has preventive and therapeutic effects on reconstruction of pulmonary vessels and pulmonary hypertension induced by high pulmonary blood flow volume.

关 键 词：L-精氨酸 肺动脉高压 左向右分流 预防 治疗 动物实验 

分 类 号：R543.3[医药卫生—心血管疾病] R965[医药卫生—内科学]