帕金森病炎症/免疫异常细胞模型的建立  

Inflammatory/immune in vitro model of Parkinson' s disease

作　　者：周慧芳[1] 薛冰[1] 李艳[1] 牛东滨[1] 王晓民[1] 

机构地区：[1]北京大学神经科学研究所,北京100083

出　　处：《中华神经医学杂志》2003年第1期48-51,78,共5页Chinese Journal of Neuromedicine

基　　金：国家重点基础研究发展规划(973)-脑功能与脑重大疾病基础研究项目(1999054000);国家自然科学基金资助项目(30271494)

摘　　要：目的观察小胶质细胞激活后形态和功能的变化,以探讨活化的小胶质细胞对多巴胺能神经元产生损伤作用的可能机制,阐明帕金森病发病的免疫机制。方法建立原代小胶质细胞培养、筛选和鉴定的方法,以细菌细胞壁脂多糖为工具药激活小胶质细胞,通过免疫组化、MTT、ELISA等方法观察小胶质细胞形态、数量和功能的变化。结果LPS激活的小胶质细胞体积增大,OX-42表达上调,释放一氧化氮(Nitric Oxide,NO)、合成超氧阴离子(O2-)及分泌细胞因子TNF-α量显著增多,而细胞数量无明显改变。结论激活的小胶质细胞对多巴胺能神经元的损伤作用,可能与释放NO、O2-及细胞因子TNF-α等细胞毒性物质有关。Objective To investigate the inflammatory mechanisms of Parkinson's disease by observing the morphological and functional changes of activated microglia. Methods The methods of culturing, selecting and verifying the primary microglial cells were decided. Microglia were activated with LPS and then immunocytochemistry, MTT and ELISA were applied to the observation of the morphological characters, quantity and functional change of microglia. Results After treatment with LPS, it was observed that microglia had an obvious morphological change. The level of NO and superoxide enhanced. Cytokines such as TNF-αreleased from microglia increased greatly. But their quantity kept stable. Conclusion Activated microglia can produce many cytotoxic factors such as NO, superoxide and proinflammatory cytokines, which may be involved in the neurodegeneration.

关 键 词：帕金森病 小胶质细胞 免疫异常 细胞因子 一氧化氮 

分 类 号：R-332[医药卫生]