脑缺血后大鼠海马CA1区神经元持续钠电流变化的研究  

Effect of cerebral ischemia on the persistent sodium current of rat CA1hippocampal neurons

作　　者：张一 周岱[1] 庄心良[2] 焦志华[3] 王士雷[3] 颜涛[3] 陈猛[3] 

机构地区：[1]苏州大学附属第一医院神经外科,江苏苏州215006 [2]上海市第一人民医院麻醉科,上海200080 [3]复旦大学医学院,上海200032

出　　处：《中华神经医学杂志》2003年第1期61-63,共3页Chinese Journal of Neuromedicine

摘　　要：目的研究脑缺血后大鼠海马CAl区神经元持续钠电流的变化,探讨钠通道阻滞剂对缺血脑组织保护的机理.方法酶消化法急性分离SD大鼠(10～14d)海马CA1区神经元,全细胞膜片钳技术记录脑缺血对持续钠电流的影响.结果缺血3min时持续钠电流增加到正常时的1.59+0.26倍(P＜0.05),缺血5 min时增加到正常时的2.92±0.46倍(P＜005).结论脑缺血时钠通道开放,持续钠电流增加.Objective To study the variations of persistent sodium current of CA1 hippocampal neurons following cerebral ischemia in rats. Methods Whole-cell patch-Clamp recordings were made from enzymatically isolated rat CA1 hippocampal neurons. Results3 min and 5 min after the cerebral ischemia occurs, the persistent sodium current increases to 1.59±0.26 times and 2.92±0.46 times respectively compared to the normal persistent sodium current (P<0.05) . Conclusion Persistent sodium current of rat CA1 hippocampal neurons increases on condition of cerebral ischemia.

关 键 词：脑缺血 持续钠电流 钠通道 海马 膜片钳 

分 类 号：R363[医药卫生—病理学]