人类甲型肝炎动物模型(狨猴)的研究  

A STUDY OF ANIMAL MODEL (CAMMON MARMOSET) FOR HUMAN HEPATITIS

作　　者：潘振业[1] 马东林[1] 邹勇[1] 沈菊阳 陈天培[1] 

机构地区：[1]卫生部上海生物制品研究所,上海200052

出　　处：《动物学杂志》1992年第6期27-31,共5页Chinese Journal of Zoology

摘　　要：本文报道以不同剂量的不同HAV毒株,经静脉和胃肠道分别接种普通狨猴和白须狨猴后,其血清ALT、AsT和LDH活性均见升高。对酶活性比接种前增加,大于十3SD值的6只狨猴作肝活组织检查,见有与急性病毒肝炎相一致的组织病理变化。此外,用免疫电镜法检出狨猴粪便中HAV颗粒,狨猴接种HAV后36天,血清IgM类和总抗HAV抗体均阳转。上述结果提示,普通狨猴有可能作为研究甲型肝炎的理想动物模型。This study presents the re-selts of experimental infection of human hepatitis A virus (The strains of Long-A-7 and Long-A-25, isolated in our country) by oral and intrave-nous inoculation on Saguinus mystax and Callith-rix jacchus. All the animals showed marked ele-vel higher than of the individual level of serum ALT, AST and LDH levels 16-66 days after the inoculation. Bath groups of C. jacchus and S. mystax shed the living virus in stool 7-17 days and 13-15 days respectively after the inoculation. All the animals were seropositive to HAV by 36days after inoculation. A sreies of liver needle biopsies were done at regular intervals after the anirnals showed higher levels of s閞um enzymes. Pathological lesions compatible with obvious viral hepatitis and the progress of the lesion were found in the specimens from all the animals both under microscopic and electron microscopic observations. The results of this study confirmed the sus-ceptibility of S. mystax to HAV and implied that C. jacchus might be taken as the second choice for the animal model of human hepatitis A.

关 键 词：甲型肝炎 动物模型 狨猴 

分 类 号：R－332[医药卫生]