丹酰氯柱前衍生人尿液多胺高效液相色谱(HPLC)快速测定  

High-Performance Liquid Chromatography(HPLC) Rapid Determination of Polyamines in Human Urine Following Pre-column Derivatization with Dansyl Chloride

作　　者：傅善基[1] 卢振铎[1] 邹雄[1] 王昕 

机构地区：[1]山东大学齐鲁医院临床药理研究所,济南250012 [2]国家药品管理局高分子产品质量检测中心,济南250013

出　　处：《山东生物医学工程》2002年第2期42-44,共3页Shandong Journal of Biomedical Engineering

摘　　要：建立了丹酰氯柱前衍生HPLC法测定人尿中多胺含量的方法。以己二胺为内标 ,uBondapak -C18(2 5 0× 4.6mm ,10um)为固定相 ,甲醇和水为流动相 ,梯度洗脱 ,柱温 5 0℃ ,流速 1ml/min ,荧光检测器测得腐胺 (PUT)、精脒 (SPD)和精胺(SP)三者回收率为PUT 97%、SPD 98%、SP 10 3% ,回归方程线性良好 ( 均大于 0 99) ,分析时间约 9min。该法简洁 ,快速 ,灵敏度高 ,重现性好 。A kind of high-performance liquid chromatographic(HPLC)method for the determination of polyamines in human urine was developed. This method was based on pre-column derivatization with dansyl chloride(Dns-Cl). The derivatives were separated on a u Bondapak-C 18 column(250×4.6mm,10um),and eluted with methanol and distilled water using a one-step linear gradient. The flow rate was 1ml/min and the temperature of column was 50℃. The column eluate was monitored by fluorescence detection(excitation,370nm;emission,506nm).The recoverys of ployamines were that puttescine(PUT)97%,spermindine(SPD)98%and spermine(SP)103%. All the relative coefficients were above 0.99. The analysis can be finished about 10 min. Therefore, it's a simple, rapid and sensitive method that can be helpful in determining polyamines in human urine and other biological tissues and body fluids.

关 键 词：肿瘤患者 丹酰氯柱前衍生 人尿液 多胺 高效液相色谱 快速测定 

分 类 号：R730.43[医药卫生—肿瘤] R730[医药卫生—临床医学]