草菇半纤维素酶系统的诱导、分布及初步定性  

Inducement, Distribution and Properties of Hemicellulases from Volvariella volvacea

作　　者：裴建军[1] 胡沂淮[1] 邵蔚蓝[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214036

出　　处：《无锡轻工大学学报（食品与生物技术）》2003年第1期61-64,共4页Journal of Wuxi University of Light Industry

摘　　要：以蔗糖、果胶、羧甲基纤维素或木聚糖为碳源培养草菇 (Volvariellavolvacea) ,对所产生的半纤维素酶进行初步研究发现 ,半纤维素酶系主要是由木聚糖诱导 .半纤维素酶中的木聚糖酶分布在胞外 ,木糖糖苷酶分布在胞内 ,阿拉伯糖苷酶胞内胞外都有分布 .木聚糖酶的最适反应温度为 6 0℃ ,最适反应 pH为 5 .8,在pH 5 .4～ 7.0时比较稳定 ,保温 1h酶活的半衰温度是 5 5℃ .木糖糖苷酶的最适反应温度为 5 5℃ ,最适反应 pH为 6 .6 ,在 pH 6 .6～ 7.4时比较稳定 ,保温 1h酶活的半衰温度是 5 2℃ .阿拉伯糖苷酶的最适反应温度为 6 0℃ ,最适反应pH为 5 .0 ,在 pH 4 .6～ 6 .2时比较稳定 ,保温 1h酶活的半衰温度为 6 0℃ .Volvariella volvacea was cultivated in the media with sucrose, pectin, carboxymethyl cellulose (CMC) or xylan as carbon source and hemicellulases were mainly induced by xylan. Xylanase was in the culture filtrate, xylosidase was in the hyphal filaments, arabinofuransidase was in both the culture filtrate and the hyphal filaments. The optimal conditions for xylanase were pH 5.8 at 60 ℃, it was stable over a pH range of 5.4～7.0, and had 1 h half life at 55 ℃. The optimal conditions for xylosidase were pH 6.6 at 55 ℃, it was stable over a pH range of 6.6～7.4, and had 1 h half life at 52 ℃. The optimal conditions for arabinofuransidase were pH 5.0 at 60 ℃, it was stable over a pH range of 4.6～6.2, and had 1 h half life at 60 ℃.

关 键 词：草菇 半纤维素酶 诱导 分布 

分 类 号：S646.13[农业科学—蔬菜学]