维甲酸对大肠癌患者T淋巴细胞亚群及集落形成的影响  

Effect of Retinoic Acid (RA) on the T-lymphocyte Subsets and T-cell Colony of Patients with Colorectal Cancer

作　　者：卫洪波[1] 王吉甫[2] 陈规划[2] 

机构地区：[1]中山大学附属第三医院普外科,广东广州510630 [2]中山大学附属第一医院普外科,广东广州510080

出　　处：《癌症》2003年第2期202-205,共4页Chinese Journal of Cancer

摘　　要：背景与目的:维甲酸是维生素A的衍生物,可完全或部分阻断实验性大肠癌的癌变过程,降低大肠癌的发生率。但对大肠癌患者T淋巴细胞亚群及集落形成的影响,尚未见报道。本文的目的是探索维甲酸对大肠癌患者免疫功能的影响。方法:对40例大肠癌患者进行前瞻性研究,所有患者均行大肠癌根治术。将病例随机分为维甲酸治疗组和对照组(每组20例),分别在术前及术后检测两组患者外周血中T淋巴细胞亚群、T淋巴细胞集落形成和血清免疫球蛋白水平。结果:(1)两组术后OKT3无显著性变化,而OKT4、T4/T8的增加及OKT8的下降有统计学意义(P<0.05)。两组对比,维甲酸治疗组OKT3、OKT4及T4/T8显著高于对照组,OKT8差异则无统计学意义(P>0.05)。(2)维甲酸治疗组患者T淋巴细胞集落数显著低于正常人T淋巴细胞集落数,但却显著高于对照组大肠癌患者T淋巴细胞集落数(P<0.05)。(3)术后1～3个月,维甲酸治疗组IgG、IgA显著高于对照组(P<0.05),两组IgG的差异则无统计学意义(P>0.05)。结论:大肠癌根治术后应用维甲酸,可提高患者的细胞免疫及体液免疫功能,促进免疫抑制状态的恢复。BACKGROUND & OBJECTIVE:Retinoic acid (RA) is the derivative of vitamin A. It can inhibit the carcinogenesis and reduce the morbidity of experimental colorectal cancer. However, there are few reports about the effect of RA on the T lymphocyte subsets and T cell colony of patients with colorectal cancer. This study was designed to investigate the effect of RA on the function of immune system and provide theoretical data for clinical. METHODS:Forty patients with colorectal cancer were divided into the RA treating group (n=20) and control group (n=20) for prospective study. Peripheral blood in all the patients was preoperatively and postoperatively was collected. Subsets (OKT3,OKT4,OKT8) of T lymphocyte were assayed. The ability to form T cell colony and the IgG, IgM, IgA in peripheral blood were also determined. RESULTS:(1)Comparing with preoperative in two groups,there was no significant difference on OKT3.OKT4 and ratio of T4/T8 significantly increased (P< 0 05) and OKT8 decreased progressively postoperatively (P< 0 05). Comparing in two groups, OKT3, OKT4 and T4/T8 were significantly higher in RA treating group than in the control group (P< 0 05); But OKT8 did not show difference between the two groups (P >0 05). (2)The ability to form T cell colony was significantly lower in RA treating group than in the health human group, and higher than in the control group (P< 0 05). (3)Comparing with control group, postoperative 1 to 3 months, IgG and IgM significantly increased in RA treating group (P< 0 05). IgA did not show significant difference. CONCLUSION:Applying RA after radical operation of colorectal carcinoma could promote the cellular and humoral immunity and improve immune state recover from immunosuppression in the patients with colorectal cancer.

关 键 词：维甲酸 大肠癌 T淋巴细胞亚群 T淋巴细胞集落 免疫功能 

分 类 号：R735.34[医药卫生—肿瘤]