Effect of isoverbascoside, a phenylpropanoid glycoside antioxidant, on proliferation and differentiation of human gastric cancer cell  

苯丙素苷类抗氧化剂异毛蕊花苷对人胃癌细胞生长和分化的影响(英文)

作　　者：陈瑞川 苏金华[1] 杨善民[1] 李忌[2] 王天叫[1] 周红[1] 

机构地区：[1]厦门大学生命科学学院抗癌研究中心,厦门361005 [2]兰州大学生物系,兰州中国730000

出　　处：《Acta Pharmacologica Sinica》2002年第11期997-1001,共5页中国药理学报（英文版）

基　　金：Project partially supported by Natural Science Foundation of Fujian Province, No C97108.

摘　　要：AIM: To investigate the effects of phenylpropanoid glycoside antioxidant isoverbascoside on cell proliferation and differentiation of human gastric cancer cell line MGC 803. METHODS: MGC 803 cells were treated with isoverbascoside. Its effects on cell proliferation, tumorigenicity, enzymatic activities, cell cycles, and gene expres-sion were respectively evaluated with cell counting, tumor formation assay, enzymatic assay, flow cytometer analysis, and Western blotting, with Me2SO as positive control. RESULTS: Isoverbascoside could markedly inhibit cell proliferation in dose- and time-dependent manner. Isoverbascoside 20 μmol/L strikingly suppressed cell tumorigenicity, activities of alkaline phosphatase (ALP), and lactate dehydrogenase (LDH), and caused G0/G1 arrest. The expression of G1→ S checkpoint related proteins, p53, p21/WAFl, and p16/INK4, were up-regulated after MGC 803 cells were treated with isoverbascoside 20 μmol/L for 4-8 h. Contrarily, the expression of C-myc protein was suppressed after 8 h treatment. CONCLUSION: Isoverbascoside inhibited cell proliferation, reversed cell malignant phenotypic characteristics, and consequently caused differentiation in MGC 803 cells. These effects might be associated with its activities of causing G0/G1 arrest and regulating the expression of cell cycle related proteins.目的:探讨苯丙素苷类抗氧化剂异毛蕊花苷对人胃癌MGC 803细胞生长和分化的影响.方法:以1.5％二甲基亚砜为阳性对照,采用细胞计数、肿瘤形成率实验、酶活性测试、流式细胞仪分析和West-ern blot等方法分别评价异毛蕊花苷对MGC 803细胞生长、致瘤性、碱性磷酸酶(ALP)及乳酸脱氢酶(LDH)活性、细胞周期和周期相关基因表达的影响.结果:MGC 803细胞经异毛蕊花苷处理后,细胞生长和增殖呈浓度和时间依赖性抑制;异毛蕊花苷20μmol/L可使细胞致瘤性明显受抑,胃癌细胞分化标志酶ALP和 LDH活性均呈时间依赖性下降,细胞周期表现为G_0/G_1期阻滞,G_1→S期调控点相关蛋白p53、p21/WAF1和p16/INK4表达上调,C-myc蛋白表达受抑.结论:异毛蕊花苷可明显抑制MGC 803细胞生长及逆转胃癌细胞恶性表型进而诱导分化,这可能与细胞周期G_0/G_1期阻滞及其对周期相关基因表达的调控作用有关.

关 键 词：isoverbascoside phenylpropanoid glycoside ANTIOXIDANTS cell division cell cycle cell differentiation cultured tumor cells stomach neoplasms 

分 类 号：R735.2[医药卫生—肿瘤]