华蟾素注射液中蟾毒内酯类成分含量检测  

HPLC separation and determination of bufadienolide in Cinobufacini Injection

作　　者：苏永华[1] 黄雪强[1] 张大志[2] 张亚妮[1] 谢觉民 凌昌全[1] 

机构地区：[1]上海第二军医大学长海医院中医科,上海200433 [2]上海第二军医大学药学院有机教研室,上海200433 [3]安徽金蟾药业集团,安徽淮北市235000

出　　处：《中成药》2003年第1期24-27,共4页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金青年科学基金 (No .30 2 0 0 36 4)

摘　　要：目的 :用高效液相色谱法分离测定华蟾素注射液中蟾毒灵、华蟾酥毒基、脂蟾毒配基等成分含量。方法 :醋酸乙酯分离提取华蟾素中脂溶性成分 ,利用高效液相色谱仪定量检测 ,以Econosphere C1 8柱为分析柱 ,乙腈 水 (50∶50 )为流动相 ,检测波长 2 99nm。结果 :该测定方法准确 ,分离效果好 ,灵敏度高。根据实验结果计算华蟾素注射液中各蟾毒内酯类成分含量分别为蟾毒灵 0 .333μg·mL- 1 ,华蟾酥毒基 0 .1 59μg·mL- 1 ,脂蟾毒配基 0 .1 1 0 μg·mL- 1 。结论 :华蟾素注射液中蟾毒灵浓度从理论上讲已达到有效作用浓度 ,可能是华蟾素注射液抗肿瘤有效成分之一。Objective: To determinate the content of bufalin,resibufogenin and cinobufagin in Cinobufacini Injection. Methods: Liposoluble components in cinobufacini were extracted with ethyl acetate and determined by HPLC using a C 18 column, acetonitrile water(50∶50) as a mobile phase and UV detection wavelength at 299nm. Results: The method showed the good resolution, high sensitivity, satisfactory accuracy and specificity. Quantitatively analyze results showed that the concentration of bufalin, cinobufagin and resibufogenin in Cinobufacini Injection were 0.333 μg·mL -1 , 0.159μg·mL -1 and 0.110μg·mL -1 , respectively. Conclusion: Bufalin in Cinobufacini Injection reached effective concentration, which was regarded as one of anti cancer components.

关 键 词：华蟾素 蟾毒灵 华蟾酥毒基 脂蟾毒配基 高效液相色谱法 中药 

分 类 号：R284.1[医药卫生—中药学]