双酚A对小鼠早期胚胎发育毒性的体外实验研究  

In vitro study of bisphenol-A toxicity on the early embryo development of mice

作　　者：裴新荣[1] 李勇[1] 龙鼎新[2] 陈星[1] 高丽芳[1] 陈祥贵[1] 

机构地区：[1]北京大学公共卫生学院营养与食品卫生学系,北京100083 [2]南华大学公共卫生学院卫生学教研室

出　　处：《中国生育健康杂志》2003年第1期34-37,F003,共5页Chinese Journal of Reproductive Health

基　　金：国家"973"项目(No.G19990 5 5 90 4);国家自然科学基金重点项目 (No. 3 0 0 3 0 12 0 )资助

摘　　要：目的　揭示双酚 A的发育毒性及其毒作用机制。　方法　采用植入后全胚胎培养模型 ,将 8.5日龄 Bal B/ C小鼠胚胎与含双酚 A的大鼠即刻离心血清共培养 4 8h (双酚 A终浓度为 0、4 0 .0、 6 0 .0、 80 .0、 10 0 .0 mg/ L ) ,观察双酚 A对小鼠胚胎生长发育和组织器官形态分化的影响。　结果　随着双酚 A剂量的增加 ,胚胎在体外的生长发育受影响越严重 ,呈现出明显的剂量 -反应关系。≥ 6 0 mg/ L的双酚 A可诱发卵黄囊 ( VYS)生长和血管分化不良、生长迟缓及形态分化异常 ,严重者出现体位异常及各种畸形 ,如心脏发育迟缓、心包积液等。　结论　在较高剂量下 ,双酚 A对体外培养的小鼠胚胎具有一定的毒性作用 ,其中对卵黄囊胎盘的结构和功能的影响可能是其发育毒作用的重要基础之一。Objective To evaluate the developmental toxicity of bisphenol A (BPA) and investigate the mechanism of it in vitro. Methods Whole embryo culture was used to evaluate the developmental toxicity of BPA on mice embryos in vitro.The embryos that removed from mother mice on gestational 8 5 d (GD 8 5) were cultured in immediately centrifugal serum(ICS) with a series of concentration of 0,40 0,60 0,80 0,100 0 mg/L of BPA for 48 hrs to evaluate the embryo development and morphological differentiation. Results BPA inhibited the development of embryos in vitro which showed a dose response relationship with concentrations of BPA.At high level(≥60 mg/L), BPA delayed the growth and cardiac tube differentiation of visceral yolk sac (VYS),and induced various embryo defects, including abnormal neural system, arch,optic,flexion and small limb bud,etc. Conclusion At high level,BPA has toxicity on mice embryos in vitro.The damage on structure and function of VYS might be part of teratogenic mechanisms of BPA.

关 键 词：双酚A 全胚胎培养 发育毒性 胚胎毒性 

分 类 号：R363[医药卫生—病理学]