L-谷氨酰胺动物组织电极的研制及应用  

Preparation of L-glutamine Tissue Sensor and its Application

作　　者：杨庆玲[1] 毕可万[1] 

机构地区：[1]中国科学院大连化学物理研究所,大连116012

出　　处：《分析化学》1992年第3期284-286,共3页Chinese Journal of Analytical Chemistry

摘　　要：将含有谷氨酰胺酶的猪肾组织切片固定于聚酯膜和聚碳酸酯膜之间,并与氨电极偶合制成L-谷氨酰胺传感器。线性范围10^(-4)～5×10^(-2)mol/L,响应时间5min。测量细胞培养基RPMI—1640,结果与实际值吻合。标准加入回收率98.75％。The tissue sensor consists of an ammonia sensor and immobilized porcine kidney slice sandwiched between the polyethylene terephthalate and polycarbonate membranes. A linear relationship was in the L-glutamine concentration range of 1×10^(-4)～5×10^(-2)mol/L with sample introduction period of 5 min. The cell culture medium RPMI 1640 was tested, the response was consistent with its content. The recovery rate was 98.75％.

关 键 词：L-谷氨酰胺 组织电极 谷氨酰胺酶 

分 类 号：O629.71[理学—有机化学]