毛细管气相色谱-电子捕获检测法测定早产儿脑神经节苷脂中糖的组成  

Determination of Sugar Composition in the Ganglioside of Brain of Prematre Delivery by Gas Chromatography Electron Capture Detection

作　　者：徐桂芸[1] 余兆楼[1] 常理文[1] 

机构地区：[1]中国科学院化学研究所,北京100080

出　　处：《分析化学》1992年第8期936-938,共3页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金

摘　　要：薄层色谱分离早产儿脑中神经节苷脂混合物,刮下TLC分离后的谱带,经HCl-甲醇解,三氟乙酰化后,用毛细管气相色谱电子捕获法测定了糖基的种类和相对摩尔比。证实了早产儿脑中主要的四种神经节苷脂为GM_1、GD_1、GD_(1b)和GT_(1b)。The gangliosidos isolated from brain of premature delivery were separated by thin-layer chromatography(TLC). The sugar composition in each ganglioside separeated Was determined using gas chromatography-electron capture detection after converting glycosyl in ganglioside into the o-methyl glycoside trifluoroacetyl acetate, respectively. The mainly, gan gliosides GM_1, GD_1, GD_(1a), GD_(1b) and GT_(1B) in brain of premature delivery were confirmed by TLC and sugar composition determination.

关 键 词：神经节苷脂 电子捕获检测 GC 

分 类 号：Q546[生物学—生物化学]