90分贝次声作用后大鼠血浆儿茶酚胺类物质含量的改变  

The change of catecholamine contents in plasma of rats exposed to 90dB infrasound

作　　者：赵志刚[1] 陈景藻[1] 张李燕[1] 

机构地区：[1]第四军医大学预防医学系放射医学教研室,西安710032

出　　处：《中华物理医学与康复杂志》2003年第1期27-29,共3页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：全军重点课题 (No .96Z0 4 2 )

摘　　要：目的　测定 16Hz、90分贝 (dB)次声连续作用后 ,大鼠血浆儿茶酚胺类物质含量的变化。方法　将 2 40只成年SD大鼠随机分为实验组与对照组 (每组 12 0只 ) ,实验组大鼠用 16Hz、90dB次声连续作用 1、7、14、2 1次 ,每日 1次 ,每次 2h ,对照组大鼠在同等条件下接受假性次声作用。分别于作用后 0 .5、6、12、18、2 4h测定大鼠血浆肾上腺素 (E)、去甲肾上腺素 (NE)含量。结果　大鼠血浆NE :与对照组相比 ,1次和 7次组 ,都于 0 .5h降至最低点 (P <0 .0 1) ,以后渐渐升高 ,至 2 4h达高峰 ,呈单峰曲线 ;14次组于 0 .5h升高后渐渐回落 ,至 12h降至最低点 (P <0 .0 1) ,然后复呈升高 ,至 2 4h达峰值 (P <0 .0 1) ,呈双峰曲线 ;2 1次组 ,于 12h降至最低点 (P <0 .0 1) ,然后渐渐升高 ,至 2 4h达峰值 (P <0 .0 1) ,呈单峰曲线。大鼠血浆E :与对照组相比 ,次声作用各次组 ,在 2 4h内始终低于对照组 (P <0 .0 1)。结论　次声可引起大鼠血浆E、NE含量的改变 ,改变情况与次声作用次数有关。Objective To observe the changes of level of plasma epinephrine (E) and norepinephine (NE) contents of the rats exposed to the 90dB infrasound. Methods Two hundred and twenty rats were randomly and equally divided into experiment and control groups. The rats in the experiment group were further divided into 4 subgroups equally and exposed to the infrasound (16Hz, 90dB) once a day, 2 hours a time for 1, 7, 14 and 21 days, respectively. The control group was treated the same except infrasound exposure. The plasma levels of the E and NE were then determined at 0.5, 6,12,18 and 24 hours after the last exposure. The results were compared between the experiment and the control groups. Results For rats in the group which exposed to infrasound for 1 and 7 times, the plasma NE level declined at the beginning and gradually raised, and reached the peak at 24h after the last exposure; for those exposed for 14 times, the NE levels elevated very briefly (0.5h) and then gradually declined, and reached the lowest point 12h, then it elevated again and reached the peak at 24h. As for plasma levels of E, it was always lower in the experiment group than that of the control group. Conclusion Both NE and E plasma levels changed under the influence of infrasound irradiation, and the changes were related to the times of exposure.

关 键 词：血浆 次声作用 肾上腺素 去甲肾上腺素 高效液相色谱 动物实验 噪声 儿茶酚胺类物质 

分 类 号：R363[医药卫生—病理学]