小鼠乳癌细胞辐射敏感突变株SX-9DNA双链断裂及其重接修复研究  

STUDIES ON DNA DSB AND ITS REJOINING REPAIR IN A MUTATED RADIOSENSITIVE MOUSE MAMMARY CANCER CELL LINE, SX-9

作　　者：周平坤[1] 魏康[1] 

机构地区：[1]军事医学科学院放射医学研究所

出　　处：《辐射研究与辐射工艺学报》1992年第1期44-47,共4页Journal of Radiation Research and Radiation Processing

摘　　要：本研究采用一种新的细胞DNA双链断裂检测方法——脉冲电场电泳法,检测了两株来源相同而辐射敏感性不同的细胞SR-1和SX-9的X线照射所致DNA双链断裂的产生和修复。结果表明两株细胞的DNA双链断裂产生没有差别,而辐射敏感细胞SX-9的DNA双链断裂重接修复能力明显低于SR-1细胞,本文对此结果与细胞辐射敏感性的关系进行了讨论。The intinial amount and repair of DNA double strand breaks (DSBs) in the radiosensitive cell SX-9 and wild-type cell SR-1 irradiated by X ray were detected by pulsed field gel electrophoresis. The results showed that the extent of DNA DSBs was similar in these two cell lines under same X ray doses, but the rejoining repairca pability of DNA DSBs in SX-9 cells was significantly lower than that in SR-1 cells. Results suggested that the increased radiosensitivity in SX-9 cells could be corr- elated with a defect in the repair of DNA DSBs.

关 键 词：DNA双链断裂 辐射敏感性 癌细胞 

分 类 号：R73－33[医药卫生—肿瘤]