重组肝再生增强因子、苦参素和丹参对肝脏星形细胞株IG12的作用  

Effects of Recombinant Augmenter of Liver Regeneration Protein, Oxy-matrine and Salvia Miltiorrhuza on Hepatic Stellate Cell Line IG12

作　　者：曹芝君[1] 邱德凯[1] 沈敏 陈颖 

机构地区：[1]上海第二医科大学仁济医院 [2]上海市杨浦区安图医院内科

出　　处：《上海第二医科大学学报》2002年第6期501-504,共4页Acta Universitatis Medicinalis Secondae Shanghai

摘　　要：目的 通过观察重组肝再生增强因子（rALR）。苦参素和丹参对大鼠肝脏星形细胞（HSC）株IG12增殖及细胞外基质合成的影响,以研究其抗肝纤维化的疗效。方法 利用DNA重组、转导、诱导及纯化等一系列方法制备肝再生增强因子重组蛋白,利用胶原酶灌注及密度梯度离心法分离肝星形细胞,用有限稀释法建立大鼠HSC株IG12;于体外观察rALR、苦参素和丹参作用于IG12后IG12酸性磷酸酶活力及细胞外基质合成的改变。结果rALR、苦参素和丹参均可显著抑制IG12的增殖,并选择性地抑制其细胞外基质的合成。结论rALR、苦参素和丹参均具有抗肝纤维化的作用;IG12对于抗肝纤维化药物的筛选具有很大的价值。Objective The efficacy of recombinant augmenter of liver regeneration protein (rALR) , oxymatrine and salvia miltiorrhuza on proliferation and synthesis of extracellular matrix of hepatic stellate cell line IG12 was investigated to evaluate their effects on liver fibrosis. Methods rALR was made with the techniques of DNA recombinant, transform, induction and purification. Hepatic stellate cells were isolated with collagenase perfusion and density gradient centrifugation. Hepatic stellate cell line IG12 was cloned using the limited diluted method. The effects of rALR, oxymatrine and salvia miltiorrhuza on hepatic stellate cell line IG12 were studied. Results rALR, oxymatrine and salvia miltiorrhuza inhibited efficiently the proliferation of IG12 , and reduced selectively the synthesis of extracellular matrix. Conclusion rALR, oxymatrine and salvia miltiorrhuza are effective medicines for liver fibrosis. Hepatic stellate cell line IG12 may be valuable for screening the drugs which act as anti - liver fibrosis agents.

关 键 词：肝纤维化 肝脏星形细胞株 苦参素 丹参 重组肝再生增强因子 疗效 抗肝纤维化药物 

分 类 号：R575.2[医药卫生—消化系统] R975.[医药卫生—内科学]