红球菌DS-3脱除二苯并噻吩中有机硫的性能初探  

Desulfurization of Dibenthiophene by Rhodococcus sp.DS-3

作　　者：马挺[1] 刘健[1] 佟明友[1] 张心平[1] 刘如林[1] 

机构地区：[1]南开大学生命科学学院,天津300071

出　　处：《微生物学报》2002年第6期726-731,共6页Acta Microbiologica Sinica

摘　　要：从孤岛油田分离到一株红球菌 (Rhodococcussp .)DS 3 ,能专一地切断二苯并噻吩(DBT)中的C—S键 ,沿 4S途径代谢 ,生成二羟联苯。实验证明 ,以 2 %的接种量脱除 5 0 μg/mLDBT底物中的硫效果最佳。在此条件下 ,适宜菌株生长和脱硫的碳源为葡萄糖 ,氮源为硝酸铵 ,初始pH为 8 2 ,生长温度为 3 0℃ ,1 5mmol/L的硫酸根离子能使其丧失脱硫能力。在上述适宜条件下 ,培养 72h后DBT中 3 4 0 4A Rhodococcus DS-3,which screened from GuDao Oil Field,can specially break the C-S bond of Dibenzothiophene (DBT),and format 2-hydrobenzophene (2-HBP).This is called 4S pathway.Here we use DS-3 seed which volume is 2%,to act on DBT substrate which concentration is about 50μg/mL.With the experiment of the degradation of DBT under the conditions of different factors,it is identified that the feasible carbon source of the growth and desulfurization of DS-3 is glucose,the feasible nitrogen source is nitrate ammonium,the compatible pH is 8.2,andthe condign growth temperature is 30℃.DS-3 may lose its ability of desulfurization under the presence of 15mmol/L SO 2- 4.DS-3 can conspicuously degraded DBT to 2-HBP,and the percentage of desulfurization reaches 34.04% in 72 hours.

关 键 词：红球菌 生物脱硫 二苯并噻吩 DBT 有机硫 燃料油 

分 类 号：X172[环境科学与工程—环境科学]