中国海南岛黎族人群Y染色体上四个微卫星基因座的多态性研究  

Y-chromosome-specific microsatellite variation in Li ethnic groups of Hainan Island, China

作　　者：李冬娜[1] 应大君[1] 区采莹[2] 陈路[2] 周贞鉴[2] 符生苗[3] 

机构地区：[1]第三军医大学解剖学教研室 [2]海南医学院 [3]海南省人民医院

出　　处：《中华医学遗传学杂志》2003年第1期46-48,共3页Chinese Journal of Medical Genetics

基　　金：国家自然科学基金 (39960 0 33)~~

摘　　要：目的　调查 4个 Y染色体短串联重复序列 (shorttandem repeat,STR)基因座的遗传多态在海南岛黎族群体中的分布状况。方法　应用多重聚合酶链反应扩增后 ,用 ABI377自动测序仪进行基因扫描分型。结果　在 2 30名无亲缘关系的黎族男性个体中观察到 4个 Y- STR基因座均有遗传多态性 ,DYS3891有 4种等位基因 ,DYS390有 5种等位基因 ,DYS391有 4种等位基因 ,DYS393有 5种等位基因。获得了黎族群体在 4个 Y- STR基因座的等位基因频率、基因多样性和单倍型频率数据。结论　为我国不同民族人群 Y染色体特异性 STR的群体遗传学、法医学提供了可比性资料 。Objective The study was conducted to reveal the distribution of genetic polymorphism of four Y chromosome specific short tandem repeat (Y specific STR) loci in Li ethnic groups in Hainan Island, China. Methods Four tetranucleotide STR loci were simultaneously amplified with fluorescently labeled primers, and genotypes were determined with an automated DNA sequencer. Results Among 230 unrelated males, the alleles at the four Y specific STR loci were composed of some complex repeat structure. 4,5,4,5 alleles were observed in loci DYS3891, DYS390, DYS391, DYS393 respectively. A set of human allele ladders for the typing of the four Y specific STRs was obtained in Li ethnic population. Gene diversity index (D) and haplotype diversity data were estimated for the four Y STRs. Conclusion The preliminary study indicates a reference population for detecting male migration events and should be useful in population genetic and forensic applications.

关 键 词：中国 海南岛 黎族人群 Y染色体 微卫星 基因痤 多态性 研究 短串联重复序列 

分 类 号：R394[医药卫生—医学遗传学]