急性白血病端粒酶逆转录酶基因表达的初步分析  

Preliminary analysis on human telomerase reverse transcriptase gene expression in acute leukemia

作　　者：张艳[1] 刘菽秋[1] 屈艺[1] 李熙鸿[2] 汪凤兰[2] 董薇[1] 刘柏林[1] 

机构地区：[1]四川大学华西基础医学与法医学院生化分子生物学教研室,成都610041 [2]华西第二医院儿科

出　　处：《中华医学遗传学杂志》2003年第1期61-63,共3页Chinese Journal of Medical Genetics

基　　金：国家自然科学基金 (39970 836)~~

摘　　要：目的　初步探讨白血病端粒酶逆转录酶 (human telomerase reverse transcriptase,h TERT)基因表达与白血病临床特征的关系。方法应用逆转录 - PCR扩增法 ,检测白血病患者和正常人外周血样中h TERT m RNA的表达 ,并进行半定量分析。结果　 4 2例初治白血病血样中 ,h TERT m RNA表达阳性率73.81% ,表达水平 0 .6 4± 0 .2 1。 2 1例完全缓解组中 ,h TERT m RNA表达阳性率 19.0 5 % ,表达水平 0 .31± 0 .16。 4例复发病例均呈 h TERT m RNA阳性表达 ,表达水平 0 .84± 0 .0 9。 5名正常人血样中 ,有 1人呈现 h TERT m RNA低水平表达 ,表达水平 0 .10。结论　 h TERT表达异常与白血病的发生发展密切相关 ,可作为白血病细胞的标志物用于白血病临床分析、病情监测。Objective: To explore the relationship between gene expression of human telomerase reverse transcriptase (hTERT) and its clinical characteristics in leukemia. Methods: The protocol of RT-PCR was used to detect the hTERTmRNA expressing levels in peripheral blood samples from leukemic patients under primary treatment (n = 42), in complete remission (n = 21), with recurrent leukemia (n = 4), and from normal subjects (n = 5), respectively. Results: The positive percentage of hTERTmRNA expression was 73.81% for the primary treatment cases, and 19.05% for the complete remission cases. All of the recurrent cases gave positive results. One of the normal controls presented low level of hTERTmRNA expression. The expressing level of hTERTmRNA in primary treatment cases was 0.64± 0.21, in complete remission leukemia 0.31±0.16, in recurrent cases 0.84±0.09, and in normal controls 0.10. Conclusion: The activation of telomerase may be an essential factor in the development of leukemia and usually be the late event in its progression. As an indicator of leukemia cell, the detection of hTERT mRNA may be used in clinical analysis, disease monitoring and prognosis judgement.

关 键 词：急性白血病 端粒酶逆转录酶 基因表达 临床分析 

分 类 号：R733.71[医药卫生—肿瘤]