它莫西芬对绒毛膜癌细胞株JAR补体调节蛋白表达的影响  

Tamoxifen Modulates the Expression of Complement Inhibitory Proteins on Choriocarcinoma Cell Line JAR

作　　者：辛云碧[1] 陈松华[2] 归绥琪[1] 葛锡锐[2] 

机构地区：[1]复旦大学附属妇产科医院,上海200011 [2]中国科学院上海生化细胞学研究所,上海200031

出　　处：《上海免疫学杂志》2003年第1期20-23,共4页Shanghai Journal of Immunology

摘　　要：为探索抗肿瘤药物Tamoxifen对绒毛膜癌细胞株JAR表面补体调节蛋白表达的影响 ,实验以绒毛膜癌细胞株JAR为研究对象 ,用流式细胞术和RT PCR分别检测Tamoxifen作用前后JAR细胞补体调节蛋白在蛋白质和mRNA水平的表达。结果显示 :在 10 6MTamoxifen作用 3d后 ,JAR细胞补体调节蛋白DAF和CD5 9的蛋白质表达水平明显降低 ,而MCP无明显改变 ;在 10 6MTamoxifen作用 2 4h后 ,JAR细胞DAF和CD5 9mRNA表达水平分别降低 88%和 6 0 %。提示Tamoxifen可能通过降低DAF和CD5 9的表达 。This study was designed to elucidate the role of anti tumor drug Tamoxifen in the expression of complement inhibitory proteins MCP,DAF and CD59 on choriocarcinoma cell line JAR JAR cells were cultured in vitro,stimulated with Tamoxifen for different time,and the expression of complement inhibitory proteins in protein and mRNA level were analyzed by flow cytometry and RT PCR Treated with Tamoxifen,the expression of complement inhibitory proteins on JAR in protein and mRNA level were significantly decreased Our study demonstrated that down regulation of the expression of complement inhibitory proteins may be a new mechanism of Tamoxifen to suppress tumor growth by enhancing the sensitivity of malignant cells to the host complement attack

关 键 词：TAMOXIFEN 补体调节蛋白 绒毛膜癌 JAR 

分 类 号：R392.6[医药卫生—免疫学]