c-raf-1反义寡核苷酸对人卵巢癌细胞放射敏感性的影响  

Effect of c-raf-1 antisense oligodeoxynucleotides on radiosensitivity of human ovarian cancer cell line

作　　者：任庆兰[1] 李少林[1] 

机构地区：[1]重庆医科大学基础医学院,重庆400016

出　　处：《实用肿瘤杂志》2003年第1期27-29,共3页Journal of Practical Oncology

基　　金：自然科学基金资助项目 (编号 3 0 0 70 2 3 0 )

摘　　要：目的　探讨癌基因 c- raf- 1的反义寡核苷酸对人卵巢癌细胞放射敏感性的影响。方法　设立 c- raf- 1正义寡核苷酸组和非处理组 (仅加入等量的脂质体 )作对照 ,用 RT- PCR检测卵巢癌细胞处理前后 c- raf- 1表达水平的变化。用平板克隆形成试验检测人卵巢癌细胞株 SKOV3在脂质体 - c- raf- 1反义寡核苷酸作用前后受 6 0 Coγ射线不同剂量照射后的集落形成率。结果　 c- raf- 1反义寡核苷酸能抑制 c- raf- 1癌基因的表达 ,经脂质体介导的 c- raf- 1反义寡核苷酸作用的卵巢癌细胞经γ射线照射后其集落形成率较转染正义寡核苷酸组及单纯照射组明显下降 (P<0 .0 1) ,而转染 c- raf- 1正义寡核苷酸组的集落形成率与单纯照射组相比无明显差异 (P>0 .0 5 )。结论　 c- raf- 1反义寡核苷酸通过抑制 c- raf- 1的表达降低人卵巢癌细胞株 SKOV 3放射抗拒性。该作用可能与 c- raf-Objective To explore the effect of lipofectin c raf 1 antisense oligodeoxynucleotides on radiosensitivity of human ovarian cancer cell line. Methods The expression of c raf 1 was detected by means of RT PCR, cell response to irradiation was evaluated by the colony forming assay. Results Lipofectin c raf 1 antisense oligodeoxynucleotides(AS ODN) suppressed the expression of c raf 1, and significantly decreased the colony forming rate of human ovarian cancer cells after ionizing irradiation( P <0.01), while non treatment and the sense oligodeoxynucleotides(SODN) groups did not decrease the radio resistance level of SKOV3 cell line( P >0.05). Conclusion c raf 1 antisense oligodeoxynucleotides sensitize the SKOV3 to ionizing irradiation through decreasing the expression of c raf 1, which might be the result related to inhibition of the celluar signal transduction pathway leading to the radiation resistant phenotype.

关 键 词：卵巢肿瘤 脂质体 寡核苷酸类 反义 辐射耐受性 

分 类 号：R737.31[医药卫生—肿瘤]