喉癌组织RB基因突变及其与临床分期的关系  

Study of RB gene in carcinoma of larynx

作　　者：王斌全[1] 陈彦球[1] 温树信[1] 王建明[1] 皇甫辉[1] 李育军[1] 

机构地区：[1]山西医科大学第一医院耳鼻咽喉科,太原030001

出　　处：《临床耳鼻咽喉科杂志》2003年第2期89-91,共3页Journal of Clinical Otorhinolaryngology

摘　　要：目的 :探讨喉癌组织中RB基因突变及其与临床分期的关系。方法 :用聚合酶链式反应和单链构象多态性 (PCR SSCP)对 90例喉癌标本进行检测。结果 :90例中 ,2例发生纯合性缺失 ,12例SSCP异常电泳 ,总突变率 15 .5 % (14 / 90 ) ;Ⅰ～Ⅱ期喉癌RB基因突变 5例 (13.9% ) ,Ⅲ～Ⅳ期RB基因突变 9例 (16 .7% ) ,两者之间差异无显著性意义。结论 :RB基因突变在喉癌的发生发展中起着一定的作用 ;Objective:To probe the mutations of RB gene in laryngeal carcinoma, and the relation between it and the clinical phase. Method: The mutations were detected by PCR SSCP (polymerase chain reaction single strand conformation polymorphism). Result:In laryngeal carcinoma, the mutations were identified in 14( 15.5 %) of 90 samples, of which 2 were large deletion, 12 were abnormal in electrophoresis of SSCP. The mutations of Ⅰ～Ⅱ stage tumor were 5( 13.9 %), of Ⅲ～Ⅳ were 9( 16.7 %), there wasn't a significant difference between them. Conclusion:RB gene mutation takes part in the development of laryngeal carcinoma. But there wasn't relation between RB gene mutation and the course of laryngeal carcinoma.

关 键 词：喉肿瘤 聚合酶链反应 RB基因 

分 类 号：R739.65[医药卫生—肿瘤]