精氨酸-甘氨酸-天门冬氨酸-丝氨酸肽对人Tenon囊成纤维细胞的贴壁抑制试验观察  

A study of RGDS peptide inhibiting the adhesion of human Tenon's capsule fibroblasts

作　　者：王津津[1] 陈凤华[1] 杨永升[2] 崔云[1] 李根林[1] 

机构地区：[1]首都医科大学附属北京同仁医院,北京100730 [2]山西省眼科医院

出　　处：《眼科》2003年第1期55-57,T004,共4页Ophthalmology in China

摘　　要：目的 :研究精氨酸 甘氨酸 天门冬氨酸 丝氨酸 (Arg Gly Asp Ser ,RGDS)肽对人Tenon囊成纤维细胞与纤维连接蛋白 (FN)粘附、增殖的影响。方法 :在传代培养的人Tenon囊成纤维细胞中加入不同终浓度的RGDS(1、0 1、0 0 1g/L) ,用未贴壁细胞记数法及MTT法测定RGDS肽对人Tenon囊成纤维细胞粘附、增殖的影响。结果 :RGDS肽能抑制成纤维细胞在FN上的粘附 ,呈浓度效应特点 ,能抑制成纤维细胞的增殖 ,呈浓度及时间效应特点。结论 :在细胞水平上证实RGDS肽可阻止人Tenon囊成纤维细胞对FN的粘附和增殖 ,具有避免青光眼滤过术后瘢痕化形成的应用前景。Objective:To investigate the effects of RGDS peptide on fibroblasts cultured from human Tenon's capsule.Method:Fibroblasts from human Tenon's capsule were examined by morphology and immunochemistry methods.After adding several different doses of RGDS peptides(1?0.1?0.01 g/L),the adhesion between cultured human Tenon's capsule fibroblasts and fibronectin(FN)were observed in vitro by counting nonattached cells and by the MTT methods.Results:RGDS peptide could inhibit the adhesion betwwen fibroblasts and fibronectin(FN) in dose dependent way,and inhibit the proliferation in dose and time way.Conclusion:RGDS peptide could inhibit cellular adhesion between fibroblast and FN and proliferation of fibroblast,and it was potentially useful by preventing scar formation in the glaucoma filtration surgery.

关 键 词：RGDS肽 药理学 青光眼 细胞培养 精氨酸-甘氨酸-天门冬氨酸-丝氨酸肽Tenon囊成纤维细胞 贴壁抑制试验 

分 类 号：R775[医药卫生—眼科]