小鼠高、低转移性肝癌3号染色体微卫星不稳定性的研究  

Microsatellite instability on chromosome 3 in hepatocellular carcinomas with low and high metastatic capacity in mice

作　　者：秦芳[1] 张树辉[1] 张霞[1] 

机构地区：[1]济南军区总医院病理科,济南250031

出　　处：《胃肠病学和肝病学杂志》2003年第1期30-33,共4页Chinese Journal of Gastroenterology and Hepatology

基　　金：国家重点基础研究发展规划资助项目 (G2 0 0 0 0 1 61 0 61 );国家自然科学基金资助项目(960 0 0 79;3990 0 1 73);全军九五医学卫生科研基金项目资助 (No .89M0 83)

摘　　要：目的　了解小鼠高、低转移性肝癌细胞系Hca/16A3-F(F)和Hca/A2 -P(P)发生微卫星不稳定性 (MSI)情况 ,探讨它们与肝癌发生及转移之间的关系。方法　随机选择 3号和 4号染色体上 10个多态微卫星标记 ,采用聚合酶链式反应 -简单重复序列多态性 (SSLP)和单链构象多态性 (SSCP)方法对F和P细胞系进行分析。结果　F和P细胞系有信息的微卫星位点其等位基因与C3H小鼠相同 ,且存在多位点MSI。结论　该肝癌细胞系Aim To investigate the frequency of microsatellite instability (MSI) in murine hepatocellular carcinoma cell lines Hca/A2-P(P) and Hca/163-F(F) with high and low metastatic capacity and explore its association with occurrence and metastasis of hepatocellular carcinoma.Methods Ten microsatellite markers were randomly selected to examine F and P cells for MSI on chromosomes 3 and 4 using PCR-simple sequence length polymorphism (PCR-SSLP) and PCR single strand conformation polymorphism (PCR-SSCP) analysis.Results Allelic genes on the chromosomes in murine hepatocellular carcinoma cell lines with informative loci were parallel to those of C 3H mice.MSI was also detected in F and P cells.Conclusion MSI of chromosome 3 plays an important role in the occurrence of hepatocellular carcinoma in mice.

关 键 词：小鼠 肝癌 3号染色体 微卫星不稳定性 研究 

分 类 号：R735.7[医药卫生—肿瘤]