膀胱癌患者外周血细胞角蛋白20 mRNA检测及意义  

The clinical significance of detection of specific CK-20 mRNA in peripheral venous blood from patients with bladder carcinoma

作　　者：钟惟德[1] 胡建波[1] 蔡岳斌[1] 魏鸿蔼[1] 刘洁珍[1] 肖聪勤[1] 曾广翘[1] 戴奇山[1] 高劲松[2] 刘健康[3] 

机构地区：[1]广州医学院附属第一人民医院泌尿外科,510180 [2]中山医科大学基因诊断中心 [3]广州医学院组胚教研室

出　　处：《中华泌尿外科杂志》2003年第1期25-27,共3页Chinese Journal of Urology

基　　金：广东省科委重点攻关课题[粤计科字 ( 2 0 0 0 ) 2 6 1]

摘　　要：目的　探讨膀胱癌患者外周血中特异性细胞角蛋白 2 0 (CK 2 0 )mRNA在诊断中的意义。　方法　膀胱癌患者 91例 ,半巢式逆转录聚合酶链反应检测其外周血有核细胞成分中CK 2 0mRNA含量 ,2 5例健康人作为对照。　结果　 91例患者中 37例 (41 % )外周血CK 2 0mRNA阳性表达 ,其中证实有转移的 2 0例中 1 7例 (85 % )阳性 ;对照组 2 5例均为阴性。外周血细胞CK 2 0mRNA的阳性率在有远处转移的病例中显著升高 (P <0 .0 1 )。　结论　检测膀胱癌患者外周血中CK 2Objective To determine the diagnostic significance of detecting the specific epithelial keratin CK 20 mRNA in peripheral venous blood from patients with bladder carcinomas. Methods Reverse transcription coupled with two step polymerase chain reaction (nested RT PCR) was used to detect CK 20 mRNA expression in the peripheral blood from patients with bladder carcinomas. Results Detection of CK 20 mRNA expression was positive in 37 of 91 patients with bladder carcinoma (41%).Among 20 patients with distant metastasis,17 were positive (85%).CK 20 mRNA was not detectable in the blood samples from 25 normal individuals.The frequency of positive CK 20 mRNA expression was signficantly higher in those with distant metastasis. Conclusions The presence of CK 20 mRNA expression in peripheral blood may be used as an early indicator of hematogenous metastasis of bladder carcinoma cells.

关 键 词：膀胱癌 外周血 细胞角蛋白20 mRNA 检测 聚合酶链反应 肿瘤转移 诊断 

分 类 号：R737.14[医药卫生—肿瘤]