白介素-6对前列腺癌细胞生长的作用  

The effect of interlenkin 6 on the growth of LNCaP and PC-3 prostatic carcinoma cells

作　　者：张小东[1] 顾永鹏[1] 侯树坤[1] 张波[2] 

机构地区：[1]北京大学人民医院泌尿外科,100044 [2]北京同仁医院泌尿外科

出　　处：《中华泌尿外科杂志》2003年第1期34-36,共3页Chinese Journal of Urology

摘　　要：目的　观察IL 6对前列腺癌细胞生长的影响 ,对雄激素依赖型和雄激素非依赖型前列腺癌细胞作用的差别。　方法　分别测定雄激素依赖的LNCaP细胞和雄激素非依赖的PC 3细胞产生IL 6的能力及外源性IL 6和抗IL 6抗体对此两种细胞生长的影响。　结果　LNCaP细胞几乎不产生IL 6 ,1ml(1 0 6 个 )PC 3细胞平均产生IL 6 1 90pg/ 4 8h ;外源性IL 6对LNCaP细胞生长有明显抑制作用 ,但对PC 3细胞的生长无明显作用 ;抗IL 6抗体能明显抑制PC 3细胞的生长 ,对LNCaP细胞生长无影响。　结论　PC 3细胞以自分泌的形式分泌大量IL 6 ,抗IL 6抗体的封闭作用可抑制PC 3细胞的生长。外源性ILObjective To investigate the effect of IL 6 on prostatic carcinoma cell lines,and differential effects on androgen dependent and androgen independent prostatic carcinoma cells. Methods The IL 6 producing capacities of LNCaP and PC 3 cells were determined,and effects of exogenous IL 6 and anti IL 6 antibodies on LNCaP and PC 3 cells were examined. Results LNCaP produced a very small amount of IL 6,but PC 3 produced more,the concentration of IL 6 being 190 pg/48h per ml(1×10 6).The exogenous IL 6 inhibited LNCaP growth significantly,but had no obvious effect on PC 3 cells.Anti IL 6 antibidies lowered PC 3 cells growth rate but had neutral effect on LNCaP. Conclusions PC 3 cells produces IL 6 massively in autocrine manner.IL 6 could be antagonized by anti IL 6 antibodies,resulting in slowing PC 3 cells growth,and LNCaP cells growth could be inhibited by exogenous IL 6.

关 键 词：前列腺癌 细胞生长 白细胞介素6 LNCAP 

分 类 号：R737.25[医药卫生—肿瘤]