三种强启动子和egt基因缺陷在重组核型多角体病毒杀虫剂研发中的意义  

The sense of three strong promoters and egt-minus in research and development of recombinant NPV pesticides

作　　者：付月君[1] 梁爱华[1] 

机构地区：[1]山西大学生物技术研究所,太原030006

出　　处：《植物保护》2003年第1期11-13,共3页Plant Protection

摘　　要：核型多角体病毒 (nuclearpolyhedrosisvirus ,NPV)是鳞翅目昆虫的重要病原微生物。采用构建重组高效表达外源毒蛋白的 p10、ph、ocu基因的强启动子 ,以提高野生型NPV的毒力 ;探讨删除杆状病毒中的蜕皮甾体尿苷二磷酸葡糖转移酶 (ecdysteroidUDP-glucosyltraansferase ,EGT)基因对改良病毒杀虫剂的杀虫效果 。Nuclear polyedrosis virus (NPV) is pathogenic to a number of lepidopteran insects. To improve the toxic effects of the wild-type NPV, this article introduces some powerful promoters-p10, ph, ocu genes' promoters, which are very important in recombinant toxin-expressing virus. The deletion of egt gene actually improves the pesticidal properties of the NPV. Insects infected by an egt-type virus display a character of reducing feeding and earlier death than these infected by wild type virus. All of these reports can be a theortical base for improving the research and use of recombinant NPV.

关 键 词：基因工程 生物杀虫剂 核型多角体病毒 EGT基因 鳞翅目昆虫 基因缺陷 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]