慢性淋巴细胞系统白血病免疫表型分析  

Analysis the immunophenotyping of chronic lymphoid leukemia

作　　者：刘艳荣[1] 常艳[1] 王卉[1] 于弘[1] 高晖[1] 陆道培[1] 陈珊珊[1] 

机构地区：[1]北京大学血液病研究所,100044

出　　处：《中华检验医学杂志》2003年第1期17-21,共5页Chinese Journal of Laboratory Medicine

摘　　要：目的　研究国内慢性淋巴细胞系统白血病的免疫表型特点。方法　采用单参数和多参数流式细胞术分析了 16 3例慢性淋巴细胞系统白血病的免疫表型。结果　 71 8% ( 117/ 16 3)患者共表达CD5和B细胞标志。采用WHO引用的计分系统 ,将病例分为B 慢性淋巴细胞白血病 (B CLL) ,毛细胞白血病 (HCL)和其他B淋巴细胞增殖性疾病。典型的B CLL表达CD5、CD2 3、CD2 0、CD19、HLA DR ,但仍有部分患者表达CD2 2、CD11c、CD2 5和FMC7。CD10 3似为HCL最特异的标志。但仅仅依靠免疫表型难以鉴别非典型B CLL、B细胞 幼淋巴细胞白血病 (B PLL)和外套细胞淋巴瘤 (MCL) ,细胞遗传学或分子生物学检查将有助于鉴别诊断。以同一标本中残存的正常淋巴细胞为内参照 ,计算前向角光散射 (FSC)指数和抗原表达指数 ,可定量地表示细胞的大小和抗原表达的强度 ,使不同的标本具有可比性。结论　免疫表型分析是诊断慢性淋巴细胞系统白血病非常有用的依据。Objective To investigate the characteristic immunophenotype of B cell chronic lymphoid leukemia in china. Method Single and multiparameter flow cytometry were used to analysis 163 cases of B cell chronic lymphoid leukemia. Results 71.8%(117/163) of cases co-expressed CD5 and B cell markers. The patients were classified into category of B cell chronic lymphocytic leukemia(B-CLL), hairy cell leukemia(HCL) and other B-cell lymphoproliferative disorders(LPDs) by using the scoring system that was recommended by world health organization (WHO). The B-CLL typically display the composite phenotypes: CD5+,CD23+,CD20+,CD19+,HLA-DR+,but the CD22,CD11c,CD25 and FMC7 were variable present in some B-CLL cases.CD103 seems the most specific marker for HCL.To differentiate diagnosis of atypical B-CLL with B-prolymphocytic leukemia(B-PLL) or mantle cell lymphoma(MCL), one must not rely exclusively on immunophenotypic dates, cytogenetic or molecular biology detection would be helpful. The index of froward scatter( FSC) and antigens expression of tumor B cells could be calculated by dividing the relevant value of residual normal T cell within same sample as internal control, so the cell size and the intensity of antigen expression could be comparable each other and quantitative between different investigations. Conclusion immunophenotypic analysis is an extremely useful adjunct in the diagnosis of chronic lymphoid leukemia.

关 键 词：白血病 淋巴细胞 免疫分型 临床诊断 

分 类 号：R733.72[医药卫生—肿瘤]