肿瘤病人T细胞核仁嗜银蛋白和辅助性T细胞亚群变化  

Changes of AgNORs expression of T lymphocytes and helper T cell subsets in peripheral blood mononuclear cells from cancer patients

作　　者：胡晓明[1] 赵武述[1] 丛笑[1] 周童亮[1] 武晓勤[1] 

机构地区：[1]中日友好医院临床医学研究所,北京100029

出　　处：《中华检验医学杂志》2003年第1期34-36,共3页Chinese Journal of Laboratory Medicine

摘　　要：目的　探讨肿瘤病人外周血单个核细胞 (PBMC)中T细胞核仁嗜银蛋白 (AgNORs)变化和辅助性T(Th)细胞亚群变化的关系及其临床意义。方法　PBMC经多克隆T细胞活化剂刺激 ,以图像分析法检测AgNORs区面积与细胞核仁面积的百分比 (I.S % ) ;PBMC被离子霉素和佛波醇酯活化 5h后 ,以酶联免疫斑点法 (ELISPOT)检测γ干扰素 (IFNγ)和白细胞介素 4(IL 4)产生细胞的频度( % ) ;以离子霉素加佛波醇酯刺激 2 4h ,用ELISA试剂盒检测培养上清液中的IFNγ 和IL 4水平。结果86例肿瘤病人和 44名正常人 ,PBMC中活化T细胞AgNORs表达分别为 ( 4 90± 1 2 0 )I S %和 ( 7 82±1 2 8)I S % ,肿瘤组下降非常显著 (P <0 0 5 ) ;肿瘤组PBMC中Th2细胞频度显著上升 ,Th/Th2比值显著下降 ;同时 ,肿瘤组PBMC产生IFNγ 和IL 4水平发生相应变化 ,变化方式与Th亚群变化相似 ;肺癌转移组与未转移组、复发组与未复发组相比 ,Th1细胞数、Th1/Th2之比和IFNγ 的产生下降非常显著 ;在肿瘤组AgNORs表达与Th1细胞频度、Th1/Th2比值、IFNγ 产生和肿瘤病人生活质量评分呈正相关。结论　肿瘤病人PBMC中T细胞表达AgNORs下降 ,与患者Th1细胞功能下降呈正相关。Objective To investigate the changes of silver-stainng protein of nuclear organizer regions(AgNORs)expression of T lymphocytes and helper T (Th) cell subsets in peripheral mononuclear cells (PBMC) from cancer patients and to analyze the relationship between them.Methods AgNORs expression in T cells activated by a multiple clonal activator was examined using a special image system. After activation of PBMC with ionomycin plus PMA for 5 h, both interferon-γ (IFN γ) and interleukin-4 (IL-4)producing cell frequencies were detected by enzyme-linked immunospot assay (ELISPOT). The stimulated with ionomycin plus PMA for 24 h, the IFN γ-and IL-4-production were assayed by ELISA.Resuts AgNORs expression of activated T cells in PBMC from cancer patients (n=86) and healthy persons (n=44) was (4.90±1.20) I.S% and (7.82±1.28) I.S% respectively. Th2 frequency was significantly higher and the ratio of Th1 to Th2 frequency was more lower in cancer patient group. Moreover, the IFN γ and IL-4 production in cancer patient group were changed in the way similar to Th subset frequencies. In lung cancer patients the AgNORs expression, Th1 frequency, Th1/Th2 ratio and IFN γ production were significantly decreased in metastasis or in recurrence status,AgNORs expression in cancer patients correlated with their Th1 frequencies, Th1/Th2 ratio, IFN γ production levels and Karnofsky′s estimators.Conclusions In analyzed cancer patient cases, the expression of AgNORs and the function of Th1 cell were significantly decreased and correlated.

关 键 词：核仁嗜银蛋白 辅助性T细胞 肿瘤 T淋巴细胞 核仁蛋白类 

分 类 号：R730.3[医药卫生—肿瘤]