慢性胃炎和消化性溃疡中幽门螺杆菌babA2和cagA及vacA基因型分析  

Analysis of babA2,cagA and vacA genotypes of Helicobacter pylori in chronic gastritis and peptic ulcer

作　　者：茅海燕[1] 方平楚[1] 叶少菁[1] 尤建飞[1] 朱永良[2] 俞建国 

机构地区：[1]浙江大学医学院病原生物学教研室,浙江杭州310031 [2]浙江大学医学院附属第二医院,浙江杭州310009 [3]浙江省舟山市岱山县人民医院,浙江舟山316200

出　　处：《浙江大学学报（医学版）》2003年第1期29-32,共4页Journal of Zhejiang University(Medical Sciences)

基　　金：CMB(96 - 6 2 8);浙江省自然科学基金 (3970 34);浙江省医药卫生科技基金 (98A0 12 )

摘　　要：目的 :研究慢性胃炎和消化性溃疡中幽门螺杆菌 (Hp) bab A2、cag A、vac A基因型的分布 ,探讨 Hpbab A2、cag A、vac A基因型与慢性胃炎和消化性溃疡的关系。方法 :用聚合酶链反应测定 5 8株从浙江省慢性胃炎和消化性溃疡患者中分离的 Hp bab A2基因、cag A基因和 vac A基因亚型。结果 :5 8株 Hp中 bab A2、cag A、vac A s1a、vac A m1、vac A m2基因的阳性率分别为 87.9%、10 0 %、93.1%、1.7%、65 .5 %。慢性胃炎和消化性溃疡患者感染的Hp bab A2、vac A s1a、vac A m2基因阳性率差异无显著性 (P>0 .0 5 )。结论 :从浙江地区慢性胃炎和消化性溃疡患者中分离的 Hp bab A2、cag A、vac A基因型均以 bab A2阳性、cag A阳性和 vac A s1a/ m2型为主 ,未发现 Hp bab A2、cag A和 vac A基因型与慢性胃炎和消化性溃疡的关系。Objective: To investigate the distribution of babA2, cagA and vacA genotypes of Helicobacter pylori (Hp) in chronic gastritis and peptic ulcer and to discuss the relationship between babA2, cagA and vacA genotypes of Hp and chronic gastritis and peptic ulcer. Methods: babA2, cagA genotypes and vacA subtype of 58 Hp strains isolated from patients of Zhejiang province with chronic gastritis or peptic ulcer were tested by polymerase chain reaction. Results: The positive rates of babA2, cagA, vacAs1a, vacA m1 and vacA m2 of 58 Hp strains were 87.9%,100%,93.1%,1.7% and 65.5%, respectively. There were no significant differences between the positive rates of babA2, vacA s1a and vacA m2 genes of Hp strains isolated from patients with chronic gastritis and peptic ulcer. Conclusion: The genotypes of Hp isolated from patients of Zhejiang province were predominatly babA2 positive, cagA positive and vacA s1a/m2. The relationships between babA2, cagA and vacA genotypes of Hp and chronic gastritis and peptic ulcer can not be identified.

关 键 词：幽门螺杆菌 基因型 胃炎 诊断 消化性溃疡 

分 类 号：R573.1[医药卫生—消化系统] R[医药卫生—内科学]