施万细胞对大鼠脊髓半横切后背核神经元存活及其表达NOS的影响  

EFFECTS OF SCHWANN CELLS ON THE NEURONAL SURVIVAL AND EXPRESSION OF NITRIC OXIDE SYNTHASE IN CLARKE’S NUCLEUS AFTER SPINAL CORD HEMISECTION IN RAT

作　　者：张燕青[1] 曾园山[1] 吴立志[1] 李海标[1] 陈穗君[1] 

机构地区：[1]中山大学中山医学院组织学胚胎学教研室神经科学研究室,广州510080

出　　处：《解剖学报》2003年第1期67-72,共6页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目 (3 0 2 70 70 0 );国家重点基础研究发展规划资助项目 (G19990 5 40 0 9)

摘　　要：目的　探讨移植的施万细胞对大鼠脊髓半横切后背核神经元存活及其表达NOS的影响。　方法　5 0只成年SD大鼠被分为实验组和对照组。在胸 11脊髓段半横切后立即在损伤处移植入施万细胞。　结果　脊髓半横切后 ,15d和 2 5d对照组L1脊髓段损伤侧背核神经元的存活数均比未损伤侧的明显减少。存活的神经元胞体出现明显皱缩 ,有些神经元呈现NADPH d阳性。 15d和 2 5d施万细胞组L1脊髓段损伤侧背核神经元的存活数则比同期对照组的明显增加 ,表达NOS的存活神经元数也随之增多。但存活的神经元胞体仍然是皱缩的。　结论　移植的SCs可促进受损伤的背核神经元存活及其表达NOS 。Objective The effects of implanted Schwann cells(SCs)on the neuronal survival and expression of nitric oxide synthase(NOS)in Clarke's nucleus(CN)following spinal cord hemisection(SCH)in rat were explored. Methods Fifty adult Sprague Dawley rats were divided into the experimental and control groups.SCs were implanted into the lesion site immediately after hemisection of T11 spinal segment. Results The survival CN neurons on lesioned side in L1 spinal segment was much less than that on intact side after SCH in 15d and 25d control groups.The soma of surviving neurons appeared obviously shrinking.Among the surviving neurons some were NADPH d positive.The survival CN neurons and their NOS expression on lesioned side in L1 spinal segment in 15d and 25d SCs groups were much more than that in 15d and 25d control groups.However,the soma of surviving neurons was still atrophy.Conclusion\ Implanted SCs may enhance the survival of injured CN neurons and NOS expression of the surviving neurons,but not prevent the soma of surviving neurons from atrophy.

关 键 词：脊髓半横切 施万细胞 背核 神经元存活 一氧化氮合酶 大鼠 脊髓外伤 

分 类 号：R651.2[医药卫生—外科学]