脑红蛋白mRNA在大鼠脑内的定位  

THE LOCALIZATION OF NGB mRNA IN ADULT RAT BRAIN

作　　者：邓美玉[1,2] 张成岗[1] 王航雁[3] 李林[1] 王春丽[1] 周婉琼[1] 

机构地区：[1]军事医学科学院放射医学研究所 [2]解放军总医院,北京100853 [3]解放军总医院

出　　处：《解剖学报》2003年第1期85-89,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金项目 (3 0 10 0 0 49);军事医学科学院科技创新启动基金资助项目 (0 10 2 0 0 1)

摘　　要：目的　了解脑红蛋白 (NGB)mRNA在大鼠脑内的定位。　方法　用地高辛标记的cRNA探针原位杂交组织化学技术观察了NGBmRNA在成年大鼠脑中的正常分布。　结果　NGBmRNA在成年大鼠脑中有非常广泛的表达 ,其分布区域包括大脑皮质、海马、丘脑、下丘脑、嗅球及小脑等。NGBmRNA阳性物质定位于神经元的细胞质。　结论　NGBmRNA在大鼠脑中有非常广泛的表达 。Objective In order to explore the localization of NGB mRNA in adult rat brain. Methods In the present study,the localization of NGB mRNA in brain of the adult rat was examined by in situ hybridization histochemistry using digoxigenin labelled cRNA probes. Results Transcripts of NGB mRNA were showed to be widely distributed throughout the adult rat brain,including cerebral cortex,hippocampus,thalamus,hypothalamus,cerebellum and olfactory bulb.Conclusion\ Our result suggested that NGB gene might play an important role in the central nervous system,possibly related to the oxygen supply of the neuron.

关 键 词：脑红蛋白 中枢神经系统 原位杂交组织化学 大鼠 

分 类 号：R364.4[医药卫生—病理学]