小鼠口服多糖包覆胰岛素脂质体的降血糖作用  

Hypoglycemic effect of polysaccharide-coated insulin liposomes after oral administration in mice

作　　者：吴正红[1] 平其能[1] 赖家明[1] 魏毅[1] 

机构地区：[1]中国药科大学药剂教研室,江苏南京210009

出　　处：《药学学报》2003年第2期138-142,共5页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目 ( 39930 2 0 0 )

摘　　要：目的　研究壳聚糖和海藻酸钠两种多糖包覆胰岛素脂质体的小鼠po降血糖作用。方法　用逆相蒸发法制备胰岛素脂质体 ;用透射电镜和激光粒度仪测定它们的形态和粒径 ;用HPLC法和超速离心法测定包封率 ;用胃蛋白酶和胰蛋白酶溶液试验多糖包覆脂质体对胰岛素的保护作用 ;用酶 苯酚法测定小鼠po多糖包覆胰岛素脂质体后降血糖作用。结果　小鼠po 0 1%壳聚糖和 0 1%海藻酸钠包覆的胰岛素脂质体具有较好的降血糖作用。Aim To evaluate the hypoglycemic effect of chitosan coated and sodium alginate coated insulin liposomes after oral administration in mice. Methods Insulin liposomes were prepared by reverse phase evaporation. Chitosan and alginate coating was carried out by mixing liposomal suspension with chitosan and sodium alginate solutions, followed by incubation. The particle size and morphology of insulin liposomes were determined using laser light scattering instrument and transmission electron microscopy (TEM). The entrapment efficiency was analyzed using HPLC and ultracentrifuge. The protection of insulin from peptic and tryptic digestion was studied with HPLC. The hypoglycemic effects of polysaccharide coated insulin liposomes were investigated using the glucose oxidase method after oral administration in mice. Results The particle size of uncoated, chitosan coated and alginate coated insulin liposomes was (138±31) nm, (230±20) nm and (266±19) nm, respectively. All insulin liposomes were of spherical or ellipsoidal shape. The entrapment efficiencies were 81 6%, 73 5% and 68 7%, respectively. Insulin was protected from tryptic digestion by chitosan coated liposomes and protected from peptic digestion by alginate coated liposomes. The hypoglycemic effects of insulin liposomes, coated with 0 1% chitosan and 0 1% sodium alginate, were observed. Conclusion Chitosan coated and sodium alginate coated liposomes were shown to reduce peptic or tryptic digestion on insulin, and enhance enteral absorption of insulin.

关 键 词：小鼠 多糖包覆 胰岛素脂质体 降血糖作用 

分 类 号：R977.15[医药卫生—药品] R965[医药卫生—药学]