Effect of dl-praeruptorin A on ATP sensitive potassium channels in human cortical neurons  

dl-白花前胡甲素对人大脑皮层ATP敏感钾通道的作用(英文)

作　　者：张沈丽[1] 李金鸣[1] 肖庆桓 吴安华 赵青[1] 杨国瑞 张克义[1] 

机构地区：[1]中国医科大学药理教研室 [2]中国医科大学第一临床学院神经外科,沈阳中国110001

出　　处：《Acta Pharmacologica Sinica》2001年第9期813-816,共4页中国药理学报（英文版）

基　　金：Project supported by the National Natural Science Foundation of China,No 39770758

摘　　要：AIM: To investigate the effect of dl-praeruptorin A (Pd-Ia) on ATP sensitive potassium channels (KATP channel) in human cortical neurons. METHODS: Using standard whole cell recording method. Cell membranes were held at - 40 mV, commanding potential was - 30 to + 100 mV and duration was 600 ms. RESULTS: Pd-Ia activated KATP channels in human cortical neurons in a concentration-dependent manner. After consecutive perfusion with external solution containing Pd-Ia 0.001, 0.01, 0.1, and 1 μmol/L, currents increased from control (0.9 ± 0.4) nA to (1.0 ± 0.4) nA, (1.1±0.4) nA, (1.2 ± 0.4) nA, and (1.3 ± 0.4) nA (P<0.05or P < 0.01, n = 5) respectively. Then the current decreased to (0.90 ± 0.37) nA (P<0.01, n=5) after washout with glibenclamide ( 10 μmol/L ). The increscent part of the currents could nearly be inhibited by specific KATP channel inhibitor. CONCLUSION: Pd-Ia could open KATP channel and it is a kind of potassium channel opener.目的:研究白花前胡甲素(Pd-Ia)对人大脑皮层神经元ATP敏感钾通道的作用。方法:应用膜片箱全细胞记录技术,采用累积给药方式。箝制电压-40mV,指令电压-30到+100mV,时程600ms。结果:Pd-Ia以浓度依赖方式激活ATP敏感钾通道。当用含不同浓度的(0.01,0.01,0.1和1μmol/L)Pd-Ia细胞外液灌流时,电流值分别从给药前的(0.9±0.4)nA增大到给药后的(1.0±0.4)nA,(1.1±0.4)nA,(1.2±0.4)nA和(1.3±0.4)nA(P<0.01,n=5)。然后用含ATP敏感钾通道的特异抑制剂格列苯肥(10μmol/L)的细胞外液冲洗,电流减小至(0.90±0.37)nA(P<0.01,n=5)。结论:Pd-Ia可开放ATP敏感钾通道,是一种钾通道开放剂。

关 键 词：potassium channels NEURONS HUMAN praeruptorin A patch-clamp techniques 

分 类 号：R285[医药卫生—中药学]