Antioxidant properties of phenolic diterpenes from Rosmarinus officinalis  

迷迭香中二萜酚化合物的抗氧化作用(英文)

作　　者：曾慧慧[1] 屠鹏飞[1] 周侃 王辉[2] 王保怀[2] 卢景芬[1] 

机构地区：[1]北京大学药学院天然药物和仿生药物国家重点实验室,北京100083 [2]北京大学物理化学研究所,北京中国100087

出　　处：《Acta Pharmacologica Sinica》2001年第12期1094-1098,共5页中国药理学报（英文版）

基　　金：National Research Laboratories of Natural and Biomimetic Drugs (No zds9604);National Natural Science Foundation of China (No 39600183)

摘　　要：AIM: To investigate the inhibition capacities of carnosol, rosmanol, and epirosmanol, which are phenolic diterpenes from Rosmarinus officinalis, to oxidized low-density lipoprotein (LDL) formation in human blood and detect their scavenging activities to lipid free radical and superoxide anion in vitro. METHODS: The anti-oxidant activities which were expressed with the inhibilities to lipid free radicals in the membrane lipid of cell and oxidized LDL formation were evaluated by TBARS assay and ESR method. The inhibition on the Cu2 +-mediated oxidization of apo B formation in LDL was investigated by fluorescence spectroscopy. RESULTS: Carnosol, rosmanol, and epirosmanol had an inhibitory activity to lipid peroxidation and oxidized apo B formation in human bloods LDL. The IC50 were 7 -10βmol/L. The antioxidant mechanism was related to the scavenging activities to lipid free radical. CONCLUSION: carnosol, rosmanol, and epirosmanol showed the activity in inhibiting LDL oxidation.目的:研究迷迭香中二萜酚化合物鼠尾草酚、迷迭香酚和表迷迭香酚对人低密度脂蛋白氧化的抑制作用及其对自由基和超氧阴离子的清除作用。方法:以细胞膜和LDL为靶,用TBARS方法和ESR方法研究了抗氧化活性。用荧光方法研究对铜诱导的LDL中apo B蛋白氧化的抑制。结果:鼠尾草酚、迷迭香酚和表迷迭香酚对人血LDL中的脂质过氧化和apo B蛋白的氧化均有抑制作用,IC_(50)值在7-10μmol/L。抗氧化机理与其对脂自由基清除活性有关。结论:鼠尾草酚、迷迭香酚和表迷迭香酚能抑制LDL氧化。

关 键 词：ANTIOXIDANTS LDL lipoproteins free radicals CARNOSOL 

分 类 号：R285[医药卫生—中药学]