Effect of anti-digoxin antiserum on endoxin and membrane ATPase activity in hypoxia-reoxygenation induced myocardial injury  

地高辛抗血清对缺氧复氧损伤心肌组织内洋地黄素和心肌细胞膜ATP酶活性的影响(英文)

作　　者：柯永胜[1] 柳兆芳[1] 杨浩[1] 杨挺 黄家胜[1] 芮世宝[1] 程光华[1] 汪佑霞[1] 

机构地区：[1]皖南医学院弋矶山医院

出　　处：《Acta Pharmacologica Sinica》2000年第4期345-347,共3页中国药理学报（英文版）

摘　　要：AIM; To evaluate the protective effect of anti-digoxin antiserum on hypoxia-reoxygenation induced injured myocardium and its mechanism. METHODS: Anti-digoxin antiserum of different concentrations was used, its effect on endoxin and ATPase activity in cell membrane in hypoxia-reoxygenation myocardium model was observed. RESULTS: The level of endoxin was remarkably higher, ATPase activities in cell membrane were remarkably lower in hypoxic group and hypoxia-reoxygenation injury group than those of normal group; anti-digoxin antiserum could resume ATPase activity in a concentration-dependent manner. CONCLUSION: Rise of endoxin was the molecular biological basis of myocardial damage during myocardial hypoxia-reoxygenation. Anti-digoxin anti-serum had lessened myocardial injury and had a protective effect on hypoxia-reoxygenation myocardium by antagonizing effect of endoxin.目的:评价地高辛抗血清对缺氧复氧损伤心肌的保护作用及其机制。方法:制备心肌组织缺氧复氧损伤模型,观察不同剂量的地高辛抗血清对缺氧复氧损伤心肌组织内洋地黄素水平和心肌细胞膜ATP酶活性的影响。结果:缺氧复氧损伤可使心肌组织内洋地黄素水平明显升高,心肌细胞膜ATP酶活性明显下降;地高辛抗血清能明显拮抗缺氧复氧对心肌细胞膜ATP酶活性的抑制作用,使酶活性得到恢复。结论:缺氧复氧损伤所致心肌组织内洋地黄素水平升高是缺氧复氧介导心肌损伤的分子生物学基础,地高辛抗血清通过拮抗内洋地黄素的作用,减轻缺氧复氧所致心肌损伤,对缺氧复氧损伤心肌具有保护作用。

关 键 词：myocardium ANOXIA ENDOXIN Na+- K+-exchanging ATPase Ca2+-transporting ATPase DIGOXIN immune sera 

分 类 号：R96[医药卫生—药理学]