肺癌易感性与NQO1、CYP1A1、mEH基因多态性的关系  

A Study on Relationship between Susceptibility to Lung Cancer and Polymorphisms of NQO1,CYP1A1,mEH Genes

作　　者：尹立红[1] 浦跃朴[1] 林嫔嫔[2] 陈文萍[3] 于力克[3] 

机构地区：[1]东南大学公共卫生学院,江苏南京210009 [2]台湾省中山医学院毒理学研究所 [3]南京市胸科医院,江苏南京210016

出　　处：《环境与职业医学》2003年第1期22-25,共4页Journal of Environmental and Occupational Medicine

基　　金：教育部高校骨干教师资助计划项目 (编号 :教技司 [2 0 0 0 ] 1 4 3);国家自然科学基金(编号 :30 1 70 791 );江苏省科技厅社会发展项目 (编号 :BS2 0 0 0 0 34)

摘　　要：[目的 ]对南京市正常人群和原发性肺癌病例进行NQO1、CYP1A1、mEH基因遗传多态性与肺癌易感性关系研究 ,探讨南京地区人群肺癌易感基因。 [方法 ]收集正常人群样本 88例 ;同时 ,应用病例 对照研究方法 ,收集南京市区原发性肺癌患者 84例 ,同时按 1∶1配对选择正常对照 84例 ,进行流行病学调查。采用PCR技术 ,对样本DNA进行NQO1、CYP1A1、mEH exon3、mEH exon4基因型的检测 ,并分析各基因型与肺癌易感性的关系。 [结果 ]南京市正常人群中 ,相关基因野生型 (wt/wt)、杂合型 (wt/vt)、突变型 (vt/vt)三种基因型的频率分布情况分别是 :NQO12 9 5 %、5 1 1%、19 3 % ;CYP1A13 5 2 %、44 3 %、2 0 5 % ;mEH exon3 2 6 1%、5 6 8%、17 0 % ;mEH exon483 0 %、15 9%、1 1%。南京市区人群NQO1、CYP1A1和mEH exon4基因多态性与肺癌易感性没有明显关系。mEH exon3基因型与肺鳞癌发生有关 ,野生型个体可降低肺鳞癌发病的风险 (OR =0 3 2 ,95 %CI :0 0 0 78～ 0 63 ) ,杂合型和突变型个体患肺鳞癌的危险性明显高于野生型个体 (OR =3 1,95 %CI :0 0 8～ 6 12 ) ;考虑吸烟因素后 ,mEH exon3基因型与吸烟者肺癌发生有关 ,野生型个体可使肺癌发病风险性降低 (OR =0 18,95 %CI :0 0 6～ 0 2 9) ,杂合型和突?Among normal population and original lung cancer patients in Nanjing,polymorphisms of NQO1,CYP1A1,mEH genes were studied in order to explore lung cancer susceptible genes in Nanjing population. 88 normal samples were collected,at the same time a case control study was conducted. 84 original lung cancer patients and 84 controls in Nanjing were chosen in match 1∶1 pair. Meanwhile,epidemiological questionnaires were conducted. Genotypes of NQO1,CYP1A1,mEH exon3,mEH exon4 genes were detected with PCR. Then,we analyzed association between genotypes and lung cancer susceptibility. The results showed that the frequency distribution of wild type,heterozygote and homozygous variant genotypes of related genes was, respectively ,as follows: NQO1 29 5%,51 1%,19 3%;CYP1A1 35 2%,44 3%,20 5%;mEH exon3 26 1%,56 8%,17 0%;mEH exon4 83 0%,15 9%,1 1%. In Nanjing population,the polymorphisms of NQO1,CYP1A1,mEH exon4 did not show a relationship with lung cancer suscepitibility. Polymorphisms in mEH exon3 were associated with lung cancer. Wild type genotype had a protective effect ( OR =0 32,95% CI : 0 007 8 ～0 63);heterozygote and homozygous variant genotypes were related to increase the risk of squamous cell carcinoma ( OR =3 1,95% CI :0 08～6 12);according to smoking state stratified,polymorphisms in mEH exon3 affected the occurrence of lung cancer in smoking individuals. Wild type genotype still showed a protective effect ( OR =0 18,95% CI :0 06～0 29);heterozygote and variant genotype were related to increase the risk of lung cancer in smoking individuals ( OR =5 66,95% CI :2 01～9 30). [Conclusion] The frequency distribution of NQO1,CYP1A1,mEH exon3,mEH exon4 genotypes differs from that of related reports in literature. The ethnic and geographic difference might be an important reason resulting in the difference in the susceptibility of lung cancer. Heterozygote and variant genotype in mEH exon3 gene associated with lung squamous cell carcinoma and

关 键 词：肺癌 NQO1基因 CYP1A1基因 mEH基因 多态性 

分 类 号：R734.202[医药卫生—肿瘤]