胶质瘤c-myc基因表达水平与其细胞增殖和凋亡关系的研究  

The relationship between c-myc gene expression and cell proliferation or apoptosis in human glioma

作　　者：于士柱[1] 浦佩玉[1] 江德华[1] 程焱[1] 安同岭[1] 管欣琴[1] 

机构地区：[1]天津医科大学总医院,天津市神经病学研究所,300052

出　　处：《现代神经疾病杂志》2003年第1期22-25,共4页

基　　金：天津市教委科研基金资助项目(98409c)

摘　　要：目的探讨胶质瘤细胞c-myc基因表达水平与肿瘤恶性程度、细胞增殖活性以及凋亡程度的关系。方法选择67例脑胶质瘤患者,其中Ⅰ～Ⅱ级者24例,Ⅲ级22例,Ⅳ级21例。应用原位杂交、原位细胞凋亡检测(TUNEL法)和免疫组织化学染色方法对其标本进行观察。结果67例患者中57例(85.1%)表达c-mycmRNA,56例(83.6%)表达c-Myc蛋白,两者表达水平呈正相关(rs=0.999,P<0.01),并均显示表达水平Ⅰ～Ⅱ级<Ⅲ级<Ⅳ级者(P<0.05或P<0.01);肿瘤细胞增殖活性和凋亡程度之间呈负相关(r=?0.775,P<0.01)。肿瘤细胞增殖活性随着肿瘤细胞c-Myc蛋白表达水平的升高而相应增强,而肿瘤细胞的凋亡则相应减少,c-Myc蛋白表达强阳性组与阳性组间,以及此两组与弱阳性组及阴性组之间比较,肿瘤细胞增殖活性和凋亡程度的差异均有显著性意义(均P<0.01)。结论c-myc基因表达水平对评价脑胶质瘤生物学行为具有临床参考价值。胶质瘤细胞c-myc基因表达异常增加可能会促进其细胞增殖和抑制其凋亡,并在胶质瘤的发生及恶性进展过程中起重要作用。Objective To study the relationship between expression level of c-myc gene and the malignant intensity, cell proliferation or apoptosis in human gliomas. Methods The specimens of sixty-seven cases of human glioma with different grades of malignancy (grade Ⅰ-Ⅱ 24 cases,Ⅲ 22 cases,Ⅳ 21 cases) were studied by means of in situ hybridization, in situ cell programmed death detection (TUNEL method) and immunohistochemical staining. Results Of the 67 gliomas 57 (85.1%) and 56 cases (83.6%) expressed c-myc mRNA and c-Myc protein respectively. The expression levels of c-myc mRNA and c-Myc protein were correlated positively with each other (rs = 0.999, P < 0.01), and the expression level increased as the tumor malignancy intensity advanced (P < 0.05 or P < 0.01). The cell proliferation activity and apoptotic degree of tumor cells were correlated negatively with each other (r = - 0.775, P < 0.01). The cell proliferation activity increased and apoptosis degree decreased when the expression level of c-Myc protein up-regulated in tumor cells. There was significant difference in tumor cell proliferation and apoptosis between the (+++) group and (++) group of c-Myc protein expression (P < 0.01) as well as between the (+++) or (++) group and (-) group or the (+) group (P < 0.01). Conclusion These results suggest that the expression level of c-myc gene has clinical reference value in the evaluation of biological behavior of glioma. Abnormal increase of c-myc gene expression in glioma cells may promote the cell proliferation and inhibit the cell apoptosis and plays an important role in the procedure of development and malignancy progression in glioma.

关 键 词：基因表达 神经胶质瘤 原癌基因蛋白质c-myc类 细胞分型 脱噬作用 

分 类 号：R739.4[医药卫生—肿瘤]