Protective effects of xanthones against myocardial ischemia-reperfusion injury in rats  

(口山)酮对大鼠缺血再灌注损伤心肌的保护作用

作　　者：姜德建[1] 谭桂山[1] 叶峰[1] 杜艳华[1] 徐康平[1] 李元建 

机构地区：[1]中南大学湘雅医学院药学院

出　　处：《Acta Pharmacologica Sinica》2003年第2期175-180,共6页中国药理学报（英文版）

摘　　要：AIM: To investigate the protective effect of xanthones against myocardial ischemia-reperfusion injury in rats.METHODS: Ischemia-reperfusion injury was induced by 20 min of global ischemia and 40 min of reperfusion in isolated rat hearts or 60-min coronary artery occlusion and 180-min reperfusion in vivo, respectively. Heart rate,coronary flow, left ventricular pressure (LVP), and its first derivative (±dp/dt_(max)) were recorded, and the activity of creatine kinase in coronary effluent and malondialdehyde contents in myocardial tissues were measured in vitro.The activity of serum creatine kinase and myocardium infarct size were measured in vivo. RESULTS: Xanthones(90 or 300 μg/L) caused a significant improvement of cardiac function (LVP and ±dp/dt_(max)) and a decrease in the release of creatine kinase in coronary effluent as well as the level of malondialdehyde in myocardial tissues. Xanthones(0.5 or 1.0 mg/kg) also markedly decreased infarct size and the release of creatine kinase in vivo. CONCLUSION:Xanthones protect the myocardium against the damages induced by ischemia-reperfusion in rats, and the effect of xanthones may be related to the inhibition of lipid peroxidation.目的:观察川东獐牙菜(口山)酮对大鼠缺血再灌注损伤心肌的保护作用。方法:离体大鼠心脏全心停灌缺血20min后再灌注40min和在体大鼠通过结扎左冠状动脉前降支1h后再灌注3h产生缺血再灌注损伤。离体心脏观测心功能(左室内压、左室内压变化最大速率±dp/dt_(max)、心率)、冠脉流量,测定肌酸激酶(CK)释放量和心肌组织丙二醛(MDA)含量;在体大鼠观测心肌梗死面积和CK释放量。结果:(口山)酮(90和300μg/L)能显著促进再灌注时心功能恢复,减少CK释放和降低MDA的含量。(口山)酮(0.5和1.0mg/kg)均可显著缩小心肌梗死面积和降低CK浓度。结论:川东獐牙菜(口山)酮具有抗心肌缺血再灌注损伤的作用,其保护作用可能与抗脂质过氧化有关。

关 键 词：XANTHONE Swertia davidi Franch myocardial reperfusion injury heart function tests lipid peroxidation 

分 类 号：R285[医药卫生—中药学]