IFN-γ对TRAIL诱导HBV相关性肝癌细胞凋亡的调节作用  

The effect of IFN-γ on TRAIL-induced apoptosis in HBV related hepatocarcinoma cell line

作　　者：韩丽辉[1] 孙汶生[1] 马春红[1] 刘素侠[1] 王晓燕[1] 于建国[2] 张利宁[1] 陈有海[3] 

机构地区：[1]山东大学医学院免疫学研究所 [2]解放军第八十八医院 [3]美国宾州大学医学院人类基因治疗研究所

出　　处：《中华微生物学和免疫学杂志》2003年第1期34-37,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金委海外青年学者合作研究基金资助项目 ( 30 12 80 2 3);国家自然科学基金资助项目 ( 30 0 70 341)

摘　　要：目的　以转染了HBV全基因组、并稳定表达HBV病毒颗粒的HepG2 .2 .15细胞为细胞模型 ,探讨IFN γ对新型凋亡分子TRAIL(TNF相关的诱导凋亡配体 )诱导凋亡的影响 ,并初步探索其发生机制。方法　以流式细胞仪检测IFN γ和TRAIL作用前后 ,HepG2 .2 .15细胞的凋亡率。分别用流式细胞术和半定量RT PCR的方法检测IFN γ作用 0、6、12和 2 4h后 ,细胞表面膜结合型TRAIL和TRAIL受体mRNA的表达。用HBsAg和HBeAgELISA检测试剂盒测定IFN γ作用 0、6、12和 2 4h后 ,HBV病毒颗粒的分泌表达状况。结果　TRAIL单独作用、IFN γ单独作用、TRAIL和IFN γ联合作用后 ,HepG2 .2 .15细胞的凋亡率分别为 9.12 %、5 .84%和 46 .6 8% ,表明IFN γ可以显著上调TRAIL诱导的细胞凋亡。IFN γ作用后 ,细胞表面膜型TRAIL的表达有显著上调 ,而部分与TRAIL诱导凋亡相关的TRAIL受体的表达也有不同程度的上调 ,并且IFN γ可以抑制HepG2 .2 .15细胞HBV病毒颗粒的分泌。结论　转染HBV全基因组的HepG2 .2 .15细胞对TRAIL诱导的凋亡相对耐受 ,而IFN γ却可以逆转这种耐受 ,使其变得对TRAIL诱导的凋亡敏感。其发生机制可能是通过IFN γ上调细胞表面TRAIL及其受体的表达 ,以及抑制HBV病毒颗粒的分泌实现的。Objective To explore the influence of IFN-γ on TRAIL (TNF related apoptosis inducing ligand)-induced apoptosis. Methods Apoptosis induced by TRAIL, IFN-γ, TRAIL plus IFN-γ was detected by flowcytometry, respectively. The expressions of membrane-bound TRAIL and its receptors were detected by flowcytometry and semi-quantitive RT-PCR respectively. The HBV particles secreted by HepG2.2.15 were detected by HBsAg and HBeAg detecting ELISA kit. Results HepG2.2.15 cell line was relatively resistant to TRAIL-induced apoptosis, but it could be made sensitive to TRAIL-induced apoptosis by IFN-γ (9.12% vesus 46.68%, P<0.01). The expression of TRAIL and some of its receptors was upregulated by IFN-γ. The amount of secreted HBV particles was decresed by IFN-γ. Conclusion IFN-γ can sensitive HepG2.2.15 cells to TRAIL-induced apoptosis to a great extent and this may be realized by the upregulated expression of TRAIL and some of its receptors and the decreased secretion of the HBV particles. [

关 键 词：IFN-Γ TRAIL HBV 肝癌 细胞凋亡 调节作用 

分 类 号：R735.7[医药卫生—肿瘤] R730.51[医药卫生—临床医学]