浓缩铀诱发人白血病细胞凋亡及相关基因调控研究  

Study on apoptosis in HL-60 cells and its related gene regulation induced by enriched uranium

作　　者：朱寿彭[1] 肖东[1] 吴越[1] 

机构地区：[1]苏州大学放射医学研究所,215007

出　　处：《中华放射医学与防护杂志》2003年第1期17-19,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家核工业基金资助项目 (Y7197Q62 0 6)

摘　　要：目的　研究浓缩铀诱发人白血病HL 6 0细胞凋亡的损伤特征及对凋亡相关基因bcl 2和bax的调控作用。方法　研究受浓缩铀内照射不同时间诱发HL 6 0细胞凋亡的DNA凝胶电泳。运用免疫组织化学技术探讨浓缩铀对HL 6 0细胞bcl 2 bax的蛋白表达 ,以及RNA分子杂交技术探讨浓缩铀对HL 6 0细胞bcl 2mRNA的转录水平表达。结果　在浓缩铀作用下 ,HL 6 0细胞的DNA呈现出在核小体间断裂的阶梯状条带形成的凋亡特征 ;并且观察到对照HL 6 0细胞中bcl 2蛋白呈高度表达 ,为 (88± 7) % ,而受浓缩铀辐照后 ,可下调至 (6 1± 5 ) %。bax在对照细胞中的表达很低 ,在浓缩铀作用下 ,未见明显改变。而且受浓缩铀辐照后 ,可使HL 6 0细胞中bcl 2mRNA表达呈明显下调。结论　浓缩铀可诱发HL 6 0细胞凋亡发生 ,其诱发凋亡的程度随浓缩铀作用时间的延长而增加。并且发现浓缩铀诱发人白血病HL 6 0细胞的凋亡作用 ,与其下调凋亡相关基因bcl 2的表达相关联。Objective\ To study characteristics of injury in apoptosis of HL\|60 cells and its related genes bcl\|2 and bax expression induced by enriched uranium 235 U. Methods\ DNA gel electrophoresis was used to show the apoptosis of HL\|60 cells.Immunohistochemical technique was used to identity the expression of bcl\|2 and bax proteins in HL\|60 cells,meanwhile RNA dot blot hybridization was used to show the expression of bcl\|2 mRNA in HL\|60 cells. Results\ HL\|60 cells irradiated with 235 U displayed characteristics of DNA ladder formation in apoptotic cells.While the expression of bcl\|2 protein was as high as (88±7)% in unirradiated control cells,down\|regulation [lowered to (61±5)%] of its expression was noted after exposure to 235 U.The expression of bax protein was low in control cells,and after irradiation it did not change obviously.A marked down\|regulation of bcl\|2 mRNA expression was found in irradiated HL\|60 cells. Conclusion\ Progression of apoptosis in HL\|60 cells induced by 235 U is dependent on the time elapse of 235 U irradiation.The apoptotic action of 235 U in HL\|60 cells is associated with down\|regulation of the apoptosis\|related gene bcl\|2.\;

关 键 词：浓缩铀^235U HL-60细胞 白血病细胞 细胞凋亡 基因调控 BCL-2基因 

分 类 号：R733.7[医药卫生—肿瘤]