大鼠坐骨神经损伤后神经与肌肉中一氧化氮合酶基因的表达  

Nitric oxide synthase expression in nerve and muscle of rat after injury of sciatic nerve

作　　者：谭湘陵[1] 陈曹逸[1] 顾晓松[2] 

机构地区：[1]江苏省南通医学院遗传学教研室,226001 [2]江苏省南通医学院分子神经生物学重点实验室,226001

出　　处：《临床神经病学杂志》2003年第1期12-15,共4页Journal of Clinical Neurology

摘　　要：目的　探讨外周神经机械损伤对神经和所支配肌肉中神经型一氧化氮合酶 (nNOS)和诱导型一氧化氮合酶 (iNOS)基因表达的影响。方法　 12只雌性SD大鼠分为 4组 ,分别用止血钳挤压右侧坐骨神经 30分钟、1、2和 5小时 ,然后取右侧坐骨神经和腓肠肌作为实验组材料 ,取左侧为自体正常对照。Trizol法提取样品总RNA。应用RT PCR法和RNA酶保护试验 (RPA)法测定 2种NOS的表达 ,以 2 ,3 二羟基丙醛 3 磷酸脱氢酶 (GAPDH)为内参。PCR电泳带和RPA杂交带的密度测定采用NIH图像分析软件。结果　与自体对照比较 ,神经组织在损伤各时间段中 2种NOS表达基本不变。肌肉组织的nNOS在 1小时组中表达升高 ,2小时组下降 ,5小时组升高。iNOS在 1和 2小时组中表达下降 ,5小时组升高。结论　神经机械损伤后神经短期内对神经自身NOS表达无影响 ,但对所支配组织中NOS表达产生影响 ,这种影响与非NOS的神经信号变化相关。Objective To observe expression of neuronal nitric oxide synthase (nNOS) and inducible NOS (iNOS) in nerve and dominated muscle of rat after injury of sciatic nerve.Methods 12 female SD rats were divided into 4 groups, right sciatic nerve was squeezed by using forceps for 0.5hr, 1hr, 2hr and 5hr respectively. Then right sciatic nerve and gastrocnemius was isolated and RNA was extracted by using Trizol reagent and meanwhile, the left sciatic nerve and gastrocnemius was used as a normal control. NOS expression was detected by using RT PCR and RNAase protection assay (RPA), and GAPDH was used as an internal standard. The density of PCR and RPA bands was determined by using NIH image software.Results 2 NOSs did not vary in nerve tissue in 4 groups, but in muscle, nNOS increased in 1h group, decreased in 2h group and increased in 5h group; iNOS decreased in 1h and 2h groups but increased in 5h group when compared to normal control.Conclusion Injuring of nerve does not effect NOS expression itself within short period, but effects the dominated muscle via the transmission of non NOS nerve signal.

关 键 词：坐骨神经损伤 坐骨神经 肌肉 一氧化氮合酶 基因表达 动物实验 

分 类 号：R363[医药卫生—病理学]