高效液相色谱法测定当归补血汤分煎液与合煎液中阿魏酸含量  

Determination of ferulic acid in Tangkuei Blood-Supplementing Decoction by HPLC

作　　者：梁光义[1] 徐必学 李霞 曹佩雪[1] 

机构地区：[1]贵州省,中国科学院天然产物化学重点实验室,贵州贵阳550002 [2]贵州省宏宇药业有限公司,贵州贵阳550002

出　　处：《中成药》2003年第2期146-148,共3页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金资助项目 ( 3996 0 0 83号 )

摘　　要：目的 :比较当归补血汤 (当归 ,黄芪 )分煎液与合煎液中阿魏酸含量。方法 :采用HypersilC1 8,以甲醇 - 0 .0 5 %乙酸(45∶55)为流动相 ,在 32 4nm波长处测定。结果 :分煎液阿魏液平均回收率为 1 0 0 .86 % ,RSD =2 .33 % ;合煎液阿魏酸平均回收率为 1 0 1 .37% ,RSD =1 .52 %。结论Objective: To compare the contents of ferulic acid in single medicine decoction (preparing the refined granules of single traditional Chinese herb from the different kinds of medicinal materials separately then mixing the granules with boiling water) and mixed medicine decoction (decocting the all kinds of the medicinal materials together as the traditional method) of Tangkuei Blood Supplementing Decoction (composed of Radix Angelicae Sinensis and Radix Astragali). Methods: HPLC conditions: Hypersil C 18 column methanol -0.05% acetic acid solution (45∶55) as mobile phases, detection wavelength at 324 nm. Results: The average recovery of single medicine decoction was 100.86% with RSD =2.33%( n =6), and that of mixed medicine decoction was 101.37% with RSD =1.52%( n =6).Conclusion: The content of ferulic acid mixed medicine decoction was higher than that in single medicine decoction.

关 键 词：当归补血汤 单味中药精制颗粒 阿魏酸 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]